Skip to main content
Addgene
Showing: 2721 - 2760 of 160228 results

  2. pET29a-YS14

    Plasmid
    #66890
    Purpose
    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)
    Depositor
    Jung-Hyun Min
    Article
    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.
    Inserts
    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    Use
    Tags
    H2A, H2B, H3, H4, His6 tag, and Thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    66890-plasmid-map-sequence-id-305064

    Enlarge

  3. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

    Enlarge

  6. CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid
    #171384
    Purpose
    Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized dCas13X.1-RESCUE-S
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171384-plasmid-map-sequence-id-337351

    Enlarge

  7. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Insert
    mTQ2=mturquoise2 blue fluorescent gene
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mutation
    Promoter
    Villin
    Available since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    67278-plasmid-map-sequence-id-115887

    Enlarge

  12. pcDNA3-Ii-OVA.I+II

    Plasmid
    #64596
    Purpose
    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation
    Depositor
    Martin Zenke, Sandra Diebold
    Article
    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.
    Insert
    Ovalbumin
    Use
    Tags
    Invariant chain
    Expression
    Mammalian
    Mutation
    Only amino acids 242-353 (class I and class II ep…
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64596-plasmid-map-sequence-id-118017

    Enlarge

  14. Anti-Glypican 4 (GPC4) [IPI-mGPC4.9] (Recombinant Antibody)

    Antibody
    #222516-rAb
    Purpose
    Anti-Glypican 4 (mouse) chimeric recombinant antibody with fused human variable and rabbit constant domains; recognizes mouse and human GPC4. Cross-reacts with mouse GPC6.
    Depositor
    Institute for Protein Innovation (IPI)
    Article
    Institute for Protein et al unpublished
    Recommended Applications
    Flow Cytometry and Immunocytochemistry
    Reactivity
    Human and Mouse
    Source Species
    Rabbit
    Isotype
    IgG1
    Trial Size
    Not available to purchase
    Available since
    Nov. 5, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Flow Cytometry image by Institute for Protein Innovation (IPI)

  18. B. subtilis CRISPRi Essential Gene Knockdown Library

    Plasmid Kit
    #1000000116
    Purpose
    B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
    Depositor
    Carol Gross
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  22. pH-ABE8e

    Plasmid
    #200774
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9(D10A)-TadA8e
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200774-plasmid-map-sequence-id-399597

    Enlarge

  25. PABE-7-NG

    Plasmid
    #138142
    Purpose
    Targeted A to G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-nCas9-NG-3XNLS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138142-plasmid-map-sequence-id-311738

    Enlarge

  28. pMSP1E2D1

    Plasmid
    #161606
    Purpose
    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Ritchie et al Methods Enzymol. 2009 . 464():211-31.
    Insert
    MSP1E2D1
    Use
    Tags
    7-His
    Expression
    Bacterial
    Mutation
    "extended" MSP1D1; contains repeat of h…
    Promoter
    T7
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161606-plasmid-map-sequence-id-318420

    Enlarge

  31. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

    Enlarge

  40. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid
    #66707
    Purpose
    expresses a single gRNA and VP64-dCas9-VP64
    Depositor
    Charles Gersbach
    Inserts
    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Expression
    Mutation
    D10A and H840A
    Promoter
    hU6 and hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66707-plasmid-map-sequence-id-244291

    Enlarge

Showing: 2721 - 2760 of 160228 results