Addgene

We narrowed to 2,310 results for: PUC19

Showing: 1361 - 1380 of 2310 results

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pML3

Plasmid

#108341

Purpose

MIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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SW Mb 64Y68F

Plasmid

#34569

Depositor

John Olson

Article

Hargrove et al J Biol Chem. 1994 Feb 11;269(6):4207-14.

Insert

SW Mb 64Y68F

Use

Tags

Expression

Bacterial

Mutation

His 64 to Tyr and Val 68 to Phe

Promoter

Available since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only
CJ41 (U1 snRNA (U1;21d))

Plasmid

#17774

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

U1 snRNA (U1;21d) (snRNA:U1:21D Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pGGC018

Plasmid

#228232

Purpose

Level 0 for LhG4 (GreenGate C module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

GR-LhG4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

-

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-Moontag:HA

Plasmid

#219648

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

MoonTag:HA SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-PGK-Blast

Plasmid

#227265

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDM042

Plasmid

#216812

Purpose

Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mScarlet3

Use

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM043

Plasmid

#216813

Purpose

Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mScarlet3

Use

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM052

Plasmid

#216814

Purpose

Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mNeonGreen

Use

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM053

Plasmid

#216815

Purpose

Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mNeonGreen

Use

Tags

RIPGLIN

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM070

Plasmid

#216816

Purpose

Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mCardinal

Use

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM071

Plasmid

#216817

Purpose

Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mCardinal

Use

Tags

RIPGLIN

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM069

Plasmid

#216821

Purpose

Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mStayGold2

Use

Tags

RIPGLIN and VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Leu2 2u

Plasmid

#220622

Purpose

empty backbone for shuttle vector construction

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Insert

empty vector (LEU2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ221

Plasmid

#212319

Purpose

Express MmFnuc guide with pureexpress

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Mercenaria guide

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ238

Plasmid

#212321

Purpose

Express KnFnuc guide with pureexpress

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFunc guide

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ217

Plasmid

#212318

Purpose

Express MmFnuc with pureexpress

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Mercenaria

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-sTagRFP-Zeo

Plasmid

#207734

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-miRFP670nano3-Zeo

Plasmid

#207735

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-C-3xFLAG-Zeo

Plasmid

#207739

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge