We narrowed to 2,364 results for: PUC19

1. pADH139

   Plasmid

   #90987

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, C, mal LEU2 2 of 2
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKE014

   Plasmid

   #207908

   Purpose

   GG entry for zCas9i expression

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   SpCas9_introns_2xNLS-Pea3A(T) term
   

   Use

   CRISPR

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKJ237

   Plasmid

   #212320

   Purpose

   Express KnFnuc

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   KnFnuc
   

   Expression

   Bacterial

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKJ226

   Plasmid

   #212316

   Purpose

   Express DpFnuc with pureexpress

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   DpFnuc
   

   Expression

   Bacterial

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAM750

   Plasmid

   #207889

   Purpose

   Level 0 for A3 module (5' TCCC-3' AACA)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   dummy 20nt sequence
   

   Use

   Synthetic Biology

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM749

   Plasmid

   #207888

   Purpose

   Level 0 for AD2 module (5' TGAC-3' TCCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   dummy 20nt sequence
   

   Use

   Synthetic Biology

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM758

   Plasmid

   #207897

   Purpose

   Level 0 for C4 module (5' GCAG-3' TCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   dummy 20nt sequence
   

   Use

   Synthetic Biology

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM756

   Plasmid

   #207895

   Purpose

   Level 0 for C2 module (5' AGCC-3' TTCG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   dummy 20nt sequence
   

   Use

   Synthetic Biology

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM757

   Plasmid

   #207896

   Purpose

   Level 0 for C3 module (5' TTCG-3' GCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   dummy 20nt sequence
   

   Use

   Synthetic Biology

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAM745

    Plasmid

    #207887

    Purpose

    Level 0 for A1 module (5' ACCT-3' TGAC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    dummy 20nt sequence
    

    Use

    Synthetic Biology

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM755

    Plasmid

    #207894

    Purpose

    Level 0 for C1 module (5' GGCT-3' AGCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    dummy 20nt sequence
    

    Use

    Synthetic Biology

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDiySpike1

    Plasmid

    #136948

    Purpose

    Plasmid for the In Vitro transcription of DiySpike1

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike1
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pR540

    Plasmid

    #207885

    Purpose

    template for 2 gRNA amplification

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    scaffold-pU6
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFPQ4

    Plasmid

    #83559

    Purpose

    Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL7 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL7

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAT15001-AncBE4max

    Plasmid

    #163016

    Purpose

    plasmid expressing AncBE4max

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT15483_e-ABE8e

    Plasmid

    #174121

    Purpose

    Expresses ABE8e fused with eCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP1698-4

    Plasmid

    #105242

    Purpose

    mammalian eGFP

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    eGFP
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIF251

    Plasmid

    #133935

    Purpose

    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.

    Depositor

    IIya Finkelstein

    Article

    Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.

    Insert

    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
    

    Use

    Λ-phage recombineering plasmid

    Available Since

    March 3, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pVD_CRISPR

    Plasmid

    #120370

    Purpose

    Plasmid containing shortened version of V-D CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_CRISPR
    

    Use

    CRISPR

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pILGFP3

    Plasmid

    #65451

    Purpose

    pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Tags

    yEGFP reporter

    Expression

    Yeast

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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