-
Plasmid#178476PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKEAP1_KELCH (KEAP1 Human, Synthetic)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pSG5-ERRbeta-delta10
Plasmid#52187PurposeExpresses human ERRbeta-delta10 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
UseTagsExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/RNAi gamma-tubulin shRNA mouse
Plasmid#70666PurposeReduced the expression of gamma-tubulin in murine cellsDepositorInsertUseU6 promoterTagsExpressionMammalianMutationPromoterU6Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pInducer21-3xFlag-S208C-MEFV
Plasmid#167019PurposeLentiviral plasmid with 3xFlag-pyrin (S208C/PAAND variant) under the control of a doxycycline-inducible promoterDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationSerine 208 changed to CysteinePromoterCMV Dox inducibleAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
BirA-Myc_N_HME1
Plasmid#167709PurposeExpresses HME1 in mammalian cellsDepositorInsertHME1 (EN1 Human)
UseTagsMyc-BirAExpressionMammalianMutationno mutationsPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-MAPKAP1RBD/Triple Mutant
Plasmid#120711PurposeExpresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 RBD
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationAmino acids 279-353, K307A, K310A, R312LPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
ETV7_A86D_pet28a
Plasmid#131657PurposeBacterial expression of ETS gene with A86D mutation for solubilityDepositorInsertETV7 A86D (ETV7 Human)
UseTags6xHisExpressionBacterialMutationAlanine 86 to Aspartic AcidPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-THAP11-rescue
Plasmid#37001DepositorInsertTHAP11 (THAP11 Human)
UseTags3xFLAGExpressionMammalianMutationMutations were generated at codons 273-275 conver…PromoterAvailable sinceMay 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
ETV3_pet28a
Plasmid#131646PurposeBacterial expression of ETS gene ETV3DepositorInsertETV3 (ETV3 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3R112C/T180CBM3
Plasmid#85090PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450 BM3
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3PromoterT7 promoterAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
RET_Y1062F-SHC
Plasmid#139620PurposeStrepIII-HA-tagged gene expressionDepositorInsertRET_Y1062F (RET Human)
UseTagsStrepIII-HAExpressionMammalianMutationY1062FPromoterCMVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
L1
Plasmid#21200DepositorUseRetroviralTagsExpressionMutationMek2 K101A fused to ER*, catalytically inactivePromoterAvailable sinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
sg resistant gamma-tubulin
Plasmid#104433PurposeFor expressing gamma-tubulin in cells expressing γTUBULIN single guide RNADepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsNoneExpressionMammalianMutationchanged t bp28 to c; g bp30 to a; c bp33 to a; …PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-Amp
Plasmid#108704PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLncEXP-lnc-BATE1
Plasmid#64864PurposeExpresses the longest isoform of lnc-BATE1DepositorInsertlnc-BATE1
UseRetroviralTagsExpressionMutationPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only