We narrowed to 165,694 results for: addgene

1. mu-FLT3Lv1-pTRIP

   Plasmid

   #163519

   Purpose

   Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

   Depositor

   Thomas Weber

   Insert

   murine-FLT3L variant 1 (Flt3l Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNR4A1.1.0-gDNA

   Plasmid

   #112391

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

   Depositor

   Kevin White

   Insert

   NR4A1 (NR4A1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PERK 7: PERK.K618A.9E10.pBABE

   Plasmid

   #21820

   Depositor

   David Ron

   Insert

   PERK K618A (Eif2ak3 Mouse)
   

   Use

   Retroviral

   Tags

   Myc (9E10)

   Expression

   Mammalian

   Mutation

   kinase dead K618A mutant

   Available Since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP

   Plasmid

   #185696

   Purpose

   AAV vector expressing cre-dependent mem-mScarlet, T2A, Synaptophysin-EGFP driven by Dlx promoter

   Depositor

   Lynette Lim

   Insert

   mScarlet, T2A, Synaptophysin-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   mScarlet palmitoylation, Synaptophysin fused to E…

   Expression

   Mammalian

   Promoter

   mDlx

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SETD1A

   Plasmid

   #32868

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETD1A (SETD1A Human)
   

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. SND1 (SND1A-c003)

   Plasmid

   #98242

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SND1A (SND1 Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00567:TUDOR 677-799

   Promoter

   T7

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. XLone-Puro NFIL3 P2A eGFP NLS

   Plasmid

   #140026

   Purpose

   Tunable and temporal expression control of NFIL3 and nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6VAH

   Plasmid

   #162273

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   TEAD2 (TEAD2 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. MSCV-FLAG-mMdm2-IRES-GFP

    Plasmid

    #66955

    Purpose

    Retroviral vector to express a FLAG-tagged mouse Mdm2

    Depositor

    Martine Roussel

    Insert

    Mdm2 (Mdm2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

11. integrin alpha9 pBabe puro

    Plasmid

    #13604

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 (ITGA9 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    contains mature ITGA9 (aa30-1035 compared to NP_0…

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

12. AAV pCAG-FLEX-mRuby3-WPRE

    Plasmid

    #99279

    Purpose

    Can be used to generate AAV virus that will express mRuby3 in the presence of Cre

    Depositor

    Rylan Larsen

    Insert

    mRuby3
    

    Use

    AAV and Cre/Lox

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe H-Ras

    Plasmid

    #18744

    Depositor

    Scott Lowe

    Insert

    H-Ras (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V

    Available Since

    July 18, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pTEAD1.1.0-gDNA

    Plasmid

    #112425

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

    Depositor

    Kevin White

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV Cbx1-V5

    Plasmid

    #175106

    Purpose

    Lentiviral expression of mouse Cbx1-V5

    Depositor

    Larry Gerace

    Insert

    Cbx1 (Cbx1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ARF1

    Plasmid

    #28168

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARF1 (ARF1 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pCMV-T7-ABEmax-SpG

    Plasmid

    #195278

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

    Depositor

    Petros Patsali, Carsten Lederer

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AD-003

    Plasmid

    #25502

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    AD-003 (NTMT1 Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. 4QPP

    Plasmid

    #61964

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Mutation

    See comments

    Promoter

    Polyhedrin

    Available Since

    Sept. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid

    #174902

    Purpose

    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Y306A, Y384F

    Promoter

    EF1

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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