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We narrowed to 5,962 results for: crispr cas9 expression plasmids

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Showing: 1361 - 1380 of 5962 results

  2. pFC332

    Plasmid
    #87845
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87845-plasmid-map-sequence-id-418925

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  3. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87844-plasmid-map-sequence-id-174620

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  4. pFC330

    Plasmid
    #87842
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    pyrG
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available Since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87842-plasmid-map-sequence-id-189665

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  5. pFC331

    Plasmid
    #87843
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87843-plasmid-map-sequence-id-177947

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  7. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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  8. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  9. pLSC-5

    Plasmid
    #62889
    Purpose
    Lenti vector for expression of inducible split Cas9
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Inserts
    SpCas9(574-1368)
    SpCas9(2-573)
    Use
    CRISPR and Lentiviral
    Tags
    FFBP12, FRB, NES PTK2, NLS SV40, and P2A
    Expression
    Mammalian
    Mutation
    *see comment below
    Promoter
    EFS
    Available Since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62889-plasmid-map-sequence-id-105912

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  12. pJAT30

    Plasmid
    #204294
    Purpose
    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Promoter
    MHC
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204294-plasmid-map-sequence-id-405490

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  13. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70708-plasmid-map-sequence-id-364684

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  15. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65779-plasmid-map-sequence-id-112660

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  19. pJAT21

    Plasmid
    #204309
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPn
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204309-plasmid-map-sequence-id-405852

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  20. pJAT25

    Plasmid
    #204310
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPc
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204310-plasmid-map-sequence-id-405854

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Showing: 1361 - 1380 of 5962 results