We narrowed to 1,670 results for: nif

1. pDL001_Hec1-ms_IgG_HC

   Plasmid

   #193618

   Purpose

   Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
   

   Tags

   His6 and signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDL002_Hec1-ms_IgG_LC

   Plasmid

   #193619

   Purpose

   Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 light chain (Hec1-ms_IgG_LC)
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. p2CT-His-MBP-Lbu_C2c2_R1079A

   Plasmid

   #83494

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1079A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1079A

   Promoter

   T7

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT024

   Plasmid

   #171636

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BFP
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBLO2 casX

   Plasmid

   #123122

   Purpose

   E. coli expression vector for CasX sgRNA

   Depositor

   Jennifer Doudna, Benjamin Oakes

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   CasX guide RNA
   

   Use

   CRISPR

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. JAB3312

   Plasmid

   #190406

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt
   

   Expression

   Plant

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. JAB3494

   Plasmid

   #190418

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter )

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter
   

   Expression

   Plant

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. JAB2602

   Plasmid

   #190389

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt
   

   Expression

   Plant

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

   Plasmid

   #87350

   Purpose

   S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/S355C/C574S/S867C

   Promoter

   T7

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

    Plasmid

    #83484

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1048A, H1053A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJCC_075 AsCas12a D908A

    Plasmid

    #171668

    Purpose

    For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a D908A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 908 to alanine

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

    Plasmid

    #83483

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. S12.1_Empty_Bridge

    Plasmid

    #127670

    Purpose

    Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. JAB2603

    Plasmid

    #190390

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt
    

    Expression

    Plant

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. S12.2_Bridge + Target

    Plasmid

    #127671

    Purpose

    Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pyc128m

    Plasmid

    #158778

    Purpose

    soluble GCaMP expression

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Empty Anchor Control

    Plasmid

    #127665

    Purpose

    Encodes for puromycin resistance expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    Puro
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJCC_058 FnCas12a D917A

    Plasmid

    #171667

    Purpose

    For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    FnCas12a D917A
    

    Tags

    6X His and sortase A sorting motif

    Expression

    Bacterial

    Mutation

    changed aspartate 917 to alanine

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR-IAA17_no stop

    Plasmid

    #83855

    Purpose

    Gateway donor vector for IAA17 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Gateway donor vector

    Mutation

    no stop codon

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS

    Plasmid

    #244837

    Purpose

    Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR

    Tags

    2xSV40 NLS

    Expression

    Mammalian

    Mutation

    G146R/R182V/E795Q

    Promoter

    T7

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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