We narrowed to 4,662 results for: crispr c plasmids

1. phABCA3-eGFP_CRISPR_Donor

   Plasmid

   #188540

   Purpose

   Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   EGFP
   

   Use

   CRISPR; Donor for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNOC-CRISPR-P2A-BlastR

   Plasmid

   #98147

   Purpose

   Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   P2A-BlastR fusion with Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-Cas3

   Plasmid

   #209427

   Purpose

   Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

   Depositor

   Tilmann Weber

   Article

   Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

   Insert

   codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; crRNA under control of PermE*

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CRISPR-Puro-sgRPL3-56

   Plasmid

   #208979

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CRISPR-Puro-sgRPL3-62

   Plasmid

   #208647

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR_RAF1_94

   Plasmid

   #111826

   Purpose

   Knockout Raf1

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting RAF1 94-114 (RAF1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQdCas12a.sggfp(C)

   Plasmid

   #236042

   Purpose

   The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPRCreb5gRNA

   Plasmid

   #195021

   Purpose

   Sequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domain

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   Creb5 gRNA (CREB5 Bovine)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPRoff-mScarletI

    Plasmid

    #217783

    Purpose

    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    

    Use

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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