-
Plasmid#201764PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T3
Plasmid#201766PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T4
Plasmid#201767PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c45
Plasmid#201771PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c46
Plasmid#201774PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c25
Plasmid#201769PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterCYC1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c15
Plasmid#201768PurposeExpresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterADH1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Trex2
Plasmid#79246PurposeExpression vector for Trex2, which is a non-processive 3' exonuclease.DepositorInsertTrex2 (Trex2 Mouse)
UseTagsExpressionMammalianMutationPromoterpCAGGSAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-R3R2
Plasmid#105109Purposecreate your own 2in1 constructsDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-R1R4
Plasmid#105110Purposecreate your own 2in1 constructsDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCASB
Plasmid#190175PurposePlasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequenceDepositorInsertsgRNA cloning site
UseTagsExpressionBacterial and YeastMutationAdded 5'-agagacc-3' upstream and 5'…PromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p16-1 hTRF2
Plasmid#12299DepositorInserthTRF2 (TERF2 Human)
UseTagsExpressionBacterialMutationA434 deleted; There is a stop codon in the 5'…PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBiFCt-2in1-NN
Plasmid#105111Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneUseTagsnYFP/cYFPExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
GI-Gal4 DBD
Plasmid#42500PurposeGIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factorDepositorInsertGIGANTEA fused with Gal4 DBD
UseTagsGal4 DNA binding domain (DBD)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-CCR5
Plasmid#222309PurposeSynchronize the trafficking of CCR5 from the ER.DepositorInsertStreptavidin-KDEL and CCR5 fused to SBP-EGFP (CCR5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-CCR5-Cys3A
Plasmid#222313PurposeSynchronize the trafficking of CCR5-Cys3A from the ER.DepositorInsertStreptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)
UseTagsExpressionMammalianMutationC321A, C323A, C324APromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBiFCt-2in1-NC
Plasmid#105112Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneUseTagscYFP-Myc and nYFP-HAExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFCt-2in1-CC
Plasmid#105114Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneUseTagscYFP-Myc/nYFP-HAExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
IRES-puro-GFP
Plasmid#16616DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBiFCt-2in1-CN
Plasmid#105113Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneUseTagscYFP-HA and nYFP-MycExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only