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pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-Puro

Plasmid

#141376

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp10 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro

Plasmid

#141381

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp15 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

Plasmid

#50834

Purpose

An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.

Depositor

Brandon Harvey

Publication

McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do

Insert

hChR2 (H134R)

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

Plasmid

#112684

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-Flpo and Nuclear localization signal

Promoter

CMV-IE

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf3b-IRES-Puro

Plasmid

#141384

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3b

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro

Plasmid

#141389

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf7b (ORF7b SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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pOttc556 - pAAV EF1a iCre

Plasmid

#210416

Purpose

AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

Depositor

Brandon Harvey, Christopher Richie

Insert

iCre

Use

AAV

Promoter

EF1a

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOttc1495 - pAAV CaMKII SERCaMP_ASARTDL

Plasmid

#192602

Purpose

An AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.

Depositor

Brandon Harvey

Insert

SERCaMP

Use

AAV

Promoter

CaMKII

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro

Plasmid

#141394

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf10 (ORF10 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

8 Articles

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

Plasmid

#141371

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

C145A, human codon optimized

Promoter

EF1alpha

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pcDNA3-3myc-6His-EZH2 21D

Plasmid

#42664

Depositor

Mien-Chie Hung

Publication

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21D

Available since

July 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1-Pur-ottlr3

Plasmid

#225641

Purpose

Expresses the Oncorhynchus tshawytscha toll-like receptor 3

Depositor

Bertrand Collet

Publication

Collet et al Mar Biotechnol (NY). 2026 Feb 27;28(2):4

Insert

Toll-like receptor 3 (tlr3 Oncorhynchus tshawytscha)

Expression

Mammalian

Available since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProTTG1 GW

Plasmid

#79753

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Martin Hulskamp

Publication

Bouyer et al PLoS Biol. 2008 Jun 10;6(6):e141. doi: 1

Type

Empty backbone

Expression

Plant

Promoter

ProTTG1

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC2234 - pAAV Nestin GFP-iCre

Plasmid

#228443

Purpose

An adeno-associated viral vector to express GFP-iCre fusion protein under the human Nestin 2nd intron enhancer.

Depositor

Christopher Richie

Publication

Ribeiro Gomes et al Cell Rep. 2025 Dec 21;45(1):116756. doi:

Insert

GFP-iCre

Use

AAV

Expression

Mammalian

Available since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro

Plasmid

#141377

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

8 Articles

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pUC57 (Gent)

Plasmid

#54338

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

Plasmid

#113162

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Tags

FLAG

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC710 - pAAV EF1a DIO Mem-AcGFP

Plasmid

#75081

Purpose

An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Publication

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Mem-AcGFP

Use

AAV and Cre/Lox

Tags

Palmitylation site

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

Plasmid

#63886

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

Depositor

Brandon Harvey

Publication

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Promoter

human SYN1

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pRK1037

Plasmid

#8833

Depositor

David Waugh

Publication

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease, catalytic domain

Expression

Bacterial

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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