-
Viral Prep#112677-AAV8PurposeReady-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)
Viral Prep#112677-AAV9PurposeReady-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)
Viral Prep#112677-AAV2PurposeReady-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH
Plasmid#131684PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1DepositorInsertsEGFP-KASH
SpCas9 sgRNA vs mouse GRID1
UseAAVTagsKASHExpressionMutationPromoterEF1a and mU6-LSL (Cre dependent)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)
Viral Prep#112677-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63885PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63887PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVTagsExpressionMutationPromoterhuman SYN1Available sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
XE51 XPKC-alpha
Plasmid#16746DepositorInsertXPKC-Alpha (prkca Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRH030
Plasmid#159633PurposeCRISPR‐Cas9 tool for the genetic engineering of Bacillus phagesDepositorTypeEmpty backboneUseCRISPR; Shuttle vectorTagsExpressionMutationPromoterConstitutive tracrRNAAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pET28a-Cys-ELP(40nm)-LPETGG
Plasmid#90475PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(40nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(40nm)-Cys
Plasmid#91571PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(40nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(120nm)-Cys
Plasmid#91572PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-HIS-CBM-Xmod-DocIII
Plasmid#91698PurposeCBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGGDepositorInsertGGG-HIS-CBM-Xmod-DocIII
UseTagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-LPETGG
Plasmid#91697PurposeCohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGGDepositorInsertCohIII-CBM-HIS-LPETGG
UseTagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMVQA
Plasmid#197894PurposepCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))DepositorInsertHIV-1 3' end (HXB2: 9045 till poly-A(10)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
DHL708
Bacterial Strain#98417PurposeBacterial Strain with Genotype: MC4100 ∆clpPXDepositorBacterial ResistanceStreptomycinAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 (Gent)
Plasmid#54338DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-BsaI
Plasmid#54340DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only