We narrowed to 18,082 results for: Pol;

1. pT7CFE1_polio_VP12A_C109R

   Plasmid

   #212987

   Purpose

   Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus Type I Mahoney VP12A C109R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   Cysteine 109 from 2A mutated to arginine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CL20_mEGFP_PICK1_POLR2F

   Plasmid

   #205894

   Purpose

   Express mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   PICK1_POLR2F (PICK1, POLR2F Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CL20_mEGFP_PICK1_POLR2F

   Plasmid

   #205894

   Purpose

   Express mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   PICK1_POLR2F (PICK1, POLR2F Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CL20_mEGFP_TTC6_MIPOL1

   Plasmid

   #205949

   Purpose

   Express mEGFP-tagged fusion protein, TTC6_MIPOL1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   TTC6_MIPOL1 (TTC6, MIPOL1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CL20_mEGFP_TTC6_MIPOL1

   Plasmid

   #205949

   Purpose

   Express mEGFP-tagged fusion protein, TTC6_MIPOL1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   TTC6_MIPOL1 (TTC6, MIPOL1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Cas9_P2A_T4pol

   Plasmid

   #190146

   Purpose

   Expresses SpCas9 and T4pol

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_T4pol
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cas9_P2A_T4pol

   Plasmid

   #190146

   Purpose

   Expresses SpCas9 and T4pol

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_T4pol
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cas9_P2A_POLB

   Plasmid

   #190145

   Purpose

   Expresses SpCas9 and POLB

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_POLB
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cas9_P2A_POLB

   Plasmid

   #190145

   Purpose

   Expresses SpCas9 and POLB

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_POLB
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPOLY_new

    Plasmid

    #179276

    Purpose

    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPOLY_new

    Plasmid

    #179276

    Purpose

    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLz58_polyPro_CsoS2_CTP

    Plasmid

    #140850

    Purpose

    Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 C-terminal peptide
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLz58_polyPro_CsoS2_CTP

    Plasmid

    #140850

    Purpose

    Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 C-terminal peptide
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLz55_polyPro_random_Npeptide

    Plasmid

    #140846

    Purpose

    Same as pLz47 but with randomized N*-peptide sequence

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with randomized CsoS2 N-peptide
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLz55_polyPro_random_Npeptide

    Plasmid

    #140846

    Purpose

    Same as pLz47 but with randomized N*-peptide sequence

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with randomized CsoS2 N-peptide
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLz56_polyPro_CsoS2_MR_r1

    Plasmid

    #140848

    Purpose

    Same as pLz47 but with MR repeat 1 instead of N*-peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 MR repeat 1
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLz56_polyPro_CsoS2_MR_r1

    Plasmid

    #140848

    Purpose

    Same as pLz47 but with MR repeat 1 instead of N*-peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 MR repeat 1
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLz47_polyPro_CsoS2_Npeptide

    Plasmid

    #140845

    Purpose

    Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 N-peptide
    

    Use

    Tags

    Hexahistidine tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLz47_polyPro_CsoS2_Npeptide

    Plasmid

    #140845

    Purpose

    Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 N-peptide
    

    Use

    Tags

    Hexahistidine tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLz57_polyPro_CsoS2_CTD_r8

    Plasmid

    #140849

    Purpose

    Same as pLz47 but with CTD repeat 8 instead of N*-peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 CTD repeat 8
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge