-
Plasmid#60736PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET:ACAT:GFP
Plasmid#60740PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET:CCCT:GFP
Plasmid#60738PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET:CACT:GFP
Plasmid#60737PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB-GST-FBXO22
Plasmid#228367Purposeexpress human FBXO22 in insect cells, such as Trichoplusia ni Hi5DepositorInsertFBXO22 (FBXO22 Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGTMV
Plasmid#118755PurposeBinary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.DepositorInsertTobacco mosaic virus
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
LRG (Lenti_sgRNA_EFS_GFP)
Plasmid#65656PurposeLentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter-driven sgRNA expression and EFS promo…Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZS1[LacI-L]
Plasmid#60745PurposeContains PI driving expression dimeric LacI, the Lactose inducible repressor.DepositorInsertLacI
UseSynthetic BiologyTagsExpressionBacterialMutationwt LacI without the tetramerization domainPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG101
Plasmid#189590PurposeTo make ATG101/ATG13 HORMA complexDepositorInsertATG101 (ATG101 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
H3S10ph biosensor
Plasmid#120809PurposeFRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRETDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
UseTagsExpressionMammalianMutationThreonine 3, Threonine 6, and Threonine 11 are mu…PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
UseTagsExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB-His-SKP1
Plasmid#228368Purposeexpress human SKP1 in insect cells, such as Trichoplusia ni Hi5DepositorInsertSKP1 (SKP1 Human)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACE-MBP-FBXL17 (310–701)
Plasmid#228369Purposeexpress human FBXL17 in insect cells, such as Trichoplusia ni Hi5DepositorInsertFBXL17 (FBXL17 Human)
UseTagsMBPExpressionInsectMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FBXL17
Plasmid#236188PurposeExpression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.DepositorInsertFLAG-FBXL17 (FBXL17 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-BACH1
Plasmid#232269PurposeExpression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.DepositorInsertBACH1 (BACH1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FFSS-BACH1
Plasmid#232273PurposeExpression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell linesDepositorInsertBACH1 (BACH1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only