We narrowed to 413 results for: pgp

1. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

   Plasmid

   #162384

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

   Plasmid

   #140197

   Purpose

   Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dLbCas12a-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNase deactivated

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CAG-VARNAM A122D WPRE-bGH-polyA

   Plasmid

   #180486

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   VARNAM A122D
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

   Plasmid

   #140193

   Purpose

   Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   dLbCas12a-VPR
   Bar
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
   

   Use

   CRISPR and Synthetic Biology; Fungal genome integ…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNAse deactivated

   Promoter

   PgpdA and PtrpC

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

   Plasmid

   #218870

   Purpose

   Mammalian expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2(no bind)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   CAG

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE

   Plasmid

   #105322

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV1

   Insert

   jGCaMP7c variant 1513
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

   Plasmid

   #105323

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7c variant 1513
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218863

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

   Plasmid

   #218867

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8f
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid

    #140198

    Purpose

    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dLbCas12a(D156R)-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNase deactivated, D156R for improved activ…

    Promoter

    PgpdA

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid

    #218865

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8s
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

    Plasmid

    #195051

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pGPD

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

    Plasmid

    #172911

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Ace2N-4AA-mNeon A122D
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid

    #172915

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7f-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

    Plasmid

    #172912

    Purpose

    Mammalian expression of voltage sensor; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Ace2N-4AA-mNeon-ST A122D
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid

    #172908

    Purpose

    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Has Service

    AAV1

    Insert

    Ace2N-4AA-mNeon-ST A122D
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    hSynapsin1

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

    Plasmid

    #172916

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7s-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid

    #172914

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7c-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

    Plasmid

    #172913

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7b-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid

    #172918

    Purpose

    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7c-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge