Addgene

We narrowed to 23,543 results for: CHI

Showing: 3451 - 3500 of 23543 results

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CAG-loxP-3xpA-tdTomato

Plasmid

#126925

Purpose

tdTomato cre reporter

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

tdTomato

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTY137 psfGFP-bimax2

Plasmid

#202410

Purpose

Expresses sfGFP-bimax2 to inhibit importin-alpha-mediated nuclear import

Depositor

Tim Mitchison

Article

Yoo et al J Cell Biol. 2021 Jul 5;220(7). pii: 212

Insert

bimax2

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pKS2

Plasmid

#24625

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

'tesA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2+8NmCherry-ABCC5a

Plasmid

#35207

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC5a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pMK-N. tabacum atpB/accD

Plasmid

#123582

Purpose

MoChlo kit

Depositor

C. Neal Stewart, Scott Lenaghan

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

N. tabacum atpB/accD integration site

Use

Chloroplast transformation vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-GLP-1

Plasmid

#66997

Purpose

Expresses malE-GLP-1 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-GLP-1

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAM-RecG-EGFP

Plasmid

#31274

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

Plasmid

#30505

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pUG-124

Plasmid

#104635

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4

Use

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdTomato-WPRE

Plasmid

#104105

Purpose

Expresses tdTomato under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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HSP104-b/Leu(WT)

Plasmid

#1156

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

GAL1:10 promoter

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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BiDi-[mEGFP]mCherry2

Plasmid

#182875

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mCherry2 and mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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SF-1

Plasmid

#28180

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

SF-1 (Nr5a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
4xmEGFP

Plasmid

#127801

Purpose

Homo-tetramer mEGFP-mEGFP-mEGFP-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mEGFP-mEGFP-mEGFP-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHH04_32_sRNA-A_Sp_coD

Plasmid

#120900

Purpose

the component plasmid of a regulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Inserts

pECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
sRNA-A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGALSc104(A503V)

Plasmid

#1152

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

A503V

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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BiDi-[mCherry2]mEGFP

Plasmid

#182874

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mEGFP, mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-SELP0K

Plasmid

#67007

Purpose

Expresses malE-SELP0K in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-SELP0K

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-PAN

Plasmid

#200971

Purpose

Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

Depositor

Yifan Cheng

Article

Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

Insert

PAN

Use

Tags

6xHis and 6xHis-thrombin site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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2xmScarlet-I

Plasmid

#127807

Purpose

Homo-dimer mScarlet-I-mScarlet-I expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mScarlet-I-mScarlet-I

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p8RwB

Plasmid

#48733

Purpose

dsRed plasmid

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

dsRed-Express

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-BRT17

Plasmid

#67000

Purpose

Expresses malE-BRT17 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-BRT17

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GPI-mGreenLantern

Plasmid

#197916

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL030

Plasmid

#53533

Purpose

supplies EtpC required for optimal EtpA expression on compatible plasmid

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpC

Use

Pacyc184

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pCS2+8NCerulean-ABCB1a

Plasmid

#34944

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCZGY2839

Plasmid

#87765

Purpose

Prgef-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Prgef-1-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEGFP(3x)

Plasmid

#182872

Purpose

Homo-trimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mEGFP(3x)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCZGY2141

Plasmid

#87757

Purpose

Pcol-19-PH::miniSOG, expression of membrane targeted PH-miniSOG in epidermis.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pcol-19-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJB005

Plasmid

#65468

Purpose

pTrc plasmid expressing GFP

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-301

Plasmid

#104730

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC10_EL3

Use

Tags

HIS-Ubq, HA-E2

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8NmCherry-ABCC1

Plasmid

#34942

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC1

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
huβB1cry:mAppleCAAX

Plasmid

#122451

Purpose

Human BB1 crystallin promoter driving mApple-CAAX in Tol2 destination vector

Depositor

Thomas Schilling

Article

Vorontsova et al J Vis Exp. 2019 May 6;(147). doi: 10.379

Insert

Human BB1 crystallin:mApple_CAAX

Use

Tags

Expression

Bacterial

Mutation

Promoter

300bp Human Beta B1 crystallin promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c5X-Dros16

Plasmid

#67004

Purpose

Expresses malE-Dros16 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Dros16

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8CmCherry-ABCG2a

Plasmid

#34943

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCG2a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pMK-S. tuberosum trnI/trnA

Plasmid

#123583

Purpose

MoChlo kit

Depositor

C. Neal Stewart, Scott Lenaghan

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

S. tuberosum trnI/trnA integration site

Use

Chloroplast transformation vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CDC12(WT)

Plasmid

#1162

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c5X-CLP3.7

Plasmid

#67011

Purpose

Expresses malE-CLP3.7 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-CLP3.7

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS404

Plasmid

#15978

Depositor

Eishi Noguchi

Article

Nakashima et al Genetics. 1999 Jul . 152(3):853-67.

Type

Empty backbone

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL-CDC12(WT)

Plasmid

#1165

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104(T499I)

Plasmid

#1150

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

T499I

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
cpi-17

Plasmid

#78708

Purpose

Encodes zebrafish cpi-17

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

cpi-17 (ppp1r14aa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
TM104b(WT)

Plasmid

#1159

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pPMS-2397

Plasmid

#231044

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to chicken Fc

Use

Affinity Reagent/ Antibody

Tags

8xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
2COX8-BmTyr

Plasmid

#222943

Purpose

mitochondrion-localized BmTyr

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

2COX8-BmTyr-Flag

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
IgK-BmTyr-KDEL

Plasmid

#222942

Purpose

ER-localized BmTyr

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

IgK-BmTyr-Flag-KDEL

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACS38

Plasmid

#204590

Purpose

GST-Progerin(506-664 delta 50)

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human progerin (506-664 delta 50)

Use

Tags

GST - progerin (506-664 delta 50)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-mCherry2-mEGFP

Plasmid

#182859

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC20_cam1-T6C

Plasmid

#182427

Purpose

Bacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1 with the T6C residue replacement to allow labelling

Depositor

Dan Mulvihill

Article

Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

Insert

cam1 (cam1 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

T6C substitution

Promoter

T7

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-mEYFP-(L)-mApple

Plasmid

#182863

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-mCherry2-(L)-mApple

Plasmid

#182858

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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