We narrowed to 4,541 results for: ALK

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pT7-V5-SBP-C1-HsGIGYF1_AI

Plasmid

#148961

Purpose

Mammalian Expression of HsGIGYF1

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1 (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-2x-Rhotekin-GBD

Plasmid

#214142

Purpose

Rho activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xRhotekin-GBD (Rtkn Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLInChR-mVenus

Plasmid

#119298

Purpose

FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

Depositor

Alla Karpova

Article

Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

Insert

Nx1BTM-FCS-ChR2ET/TC-mVenus

Use

AAV

Tags

mVenus

Promoter

CAG

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDest17_N-myc

Plasmid

#234045

Purpose

Bacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage site

Depositor

Tanja Mittag

Article

Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

Insert

N-myc (MYCN Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSC5

Plasmid

#188602

Purpose

Superior Conjugative Plasmid to Diverse Yeast

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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His-SUMO-hDDI2_pET11a

Plasmid

#169120

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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TRE-STOP EF1a-rtTA3

Plasmid

#195882

Purpose

control construct of TRE-VEGFA EF1a-rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Mouse, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His-MBP-NLS-RfxCas13d-NLS-HA

Plasmid

#171586

Purpose

Bacterial expression of NLS-RfxCas13d-NLS-HA

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

His-MBP-NLS-RfxCas13d-NLS-HA

Tags

HA

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 TGF beta type I receptor (T202D) Flag

Plasmid

#14833

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

flag

Expression

Mammalian

Mutation

Threonine 202 mutated to aspartate. Constitutivel…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pU6 SgRNA RBM20

Plasmid

#162735

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

Spacer sequence for RBM20

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

Plasmid

#182357

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

GFP

Expression

Worm

Promoter

Peft-3

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-LiMETER-v1

Plasmid

#113933

Purpose

Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

LOV2 and Rit polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-CI-SaCas9-W3SynpA

Plasmid

#231369

Purpose

minBG-driven SaCas9, with short synthetic terminator sequence.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

SaCas9

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minCMVe-SCP1-SaCas9-W3SynpA

Plasmid

#231370

Purpose

SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

SaCas9

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Article

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Promoter

hSyn

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_HcRed

Plasmid

#25892

Purpose

Gateway entry vector containing HcRed.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

hcRed

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pscAluYNF1

Plasmid

#50934

Purpose

Expresses scAluY RNA in mammalian cells.

Depositor

Katharina Strub

Article

Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

Insert

scAluYNF1

Expression

Mammalian

Mutation

the poly(A) linker and the right arm of the dimer…

Promoter

7SL RNA promoter (pol III)

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pLIC-myc-GBAi W211A

Plasmid

#171754

Purpose

For the mammalian expression of myc-GBAi W211A

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

myc

Expression

Mammalian

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0750

Plasmid

#185627

Purpose

Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

lacZ

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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