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pEJS1099: Dual-sgRNA.Design 4

Plasmid

#159537

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with two guide RNA cassettes

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-HisMBP-SED1

Plasmid

#178191

Purpose

SED1 biosensor for expression in Escherichia coli

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

6xHis-MBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-R4R3-MC1DTA

Plasmid

#139521

Purpose

Provides negative selection cassette on the vector

Depositor

Minoru Ko

Type

Empty backbone

Use

Mouse Targeting

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST47-SIRT7

Plasmid

#242137

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT7 (SIRT7 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST14-Avitag-SpyCatcher002

Plasmid

#102828

Purpose

Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Avitag-SpyCatcher002

Tags

Avitag, GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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372 pTol2-fli1ep:EGFPDest

Plasmid

#73491

Purpose

Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:EGFP-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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anti-Desmin_D7-pBIOCAM5

Plasmid

#39346

Depositor

John McCafferty

Article

Schofield et al Genome Biol. 2007;8(11):R254.

Insert

scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6-DEST PINK1-V5 WT

Plasmid

#13320

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST47-SAR1-GFP

Plasmid

#67409

Purpose

Mammalian expression of hSar1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST mfap4:tdTomato

Plasmid

#135214

Purpose

Red tagged macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter and tdTomato

Use

Zebrafish expression

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCK017 pDEST exorh:EGFP

Plasmid

#195983

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-wtSrc

Plasmid

#140295

Purpose

Expresses Flag-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Tags

2X Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDestTol2-ubi-ASAP1

Plasmid

#121987

Purpose

ubiqutously express ASAP1 voltage biosensor

Depositor

GuangJun Zhang

Article

Silic et al J Vis Exp. 2018 Apr 25;(134). doi: 10.37

Insert

ASAP1

Use

Tol2 transposon destination vector

Promoter

zebrafish ubiquitin promoter

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5/DEST-VPS35

Plasmid

#21691

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Lentiviral; Invitrogen gateway destination vector

Tags

V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST-mScarlet-SH3PXD2A

Plasmid

#215534

Purpose

Expression vector with Tks5 tagged with N-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

SH3PXD2A (SH3PXD2A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST51-LRRK2-R1441C

Plasmid

#25081

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arginine 1441 mutated to Cysteine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pEJS1096 Dual-sgRNA.Design 1

Plasmid

#159538

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 nuclease with two guide RNA cassettes with promoters

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-ARHGEF2

Plasmid

#207959

Purpose

Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

ARHGEF2 (ARHGEF2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-RNF2

Plasmid

#31216

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

RNF2 (RNF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pDESTpK7WG-RPS5Ap-mito

Plasmid

#163145

Purpose

Plant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG

Expression

Plant

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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