We narrowed to 1,529 results for: Flp

1. pDONR221-ARL5A

   Plasmid

   #67324

   Purpose

   hArl5a with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL5A (ARL5A Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Mutation

   bp 70 C to T; silent mutation

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDONR221-ARL5A-no stop

   Plasmid

   #67325

   Purpose

   hArl5a without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL5A (ARL5A Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Mutation

   bp 70 C to T; silent mutation

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pEF5/FRT/DEST-3xHA/mGli3/Flag

   Plasmid

   #51246

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEF5/FRT/DEST-3xHA/mGli2 P1-6A

   Plasmid

   #51251

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 789,805,817,848,923,956 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

   Plasmid

   #114377

   Purpose

   expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP and Kv2.1C-linker-TlcnC

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCol1a1-frt (FVB)

   Plasmid

   #63575

   Purpose

   Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

   Depositor

   Ivo Huijbers

   Article

   Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

   Insert

   Col1A-frt-hygro-pA
   

   Use

   Mouse Targeting; Frt/flpe

   Expression

   Bacterial and Mammalian

   Promoter

   None

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

   Plasmid

   #51255

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 216,220,224,230,248 mutated to Ala

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

   Plasmid

   #51257

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 216,220,224,230,248,789,805,817,848,9…

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

   Plasmid

   #51256

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 216,220,224,230,248 mutated to Glu

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pEF5/FRT/DEST-3xHA/mGli2 P1-4A

    Plasmid

    #51252

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 789,805,817,848 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pEF5/FRT/DEST-3xHA/mGli2 P5-6D

    Plasmid

    #51254

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Asp

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pEF5/FRT/DEST-3xHA/mGli2 P5-6A

    Plasmid

    #51253

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

13. CA-0176 CMVTO-mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra

    Plasmid

    #231202

    Purpose

    CageIL-10-mono (mouse renin cut-site)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra (IL10 Human)
    

    Expression

    Mammalian

    Mutation

    N36I, N110I, K117N, F129L

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-Blasticidin XLone-eGFP

    Plasmid

    #159754

    Purpose

    AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    all-in-one tet-on system
    

    Use

    CRISPR and TALEN; Donor plasmid for targeted knoc…

    Expression

    Mammalian

    Promoter

    TRE3GS inducible promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-LAP-KNL1-M3

    Plasmid

    #115896

    Purpose

    Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.

    Insert

    KNL1-M3 (KNL1 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Fragment of KNL1 containing three KNL1 repeat seq…

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Triple ORF FRT ifgMosaic Donor (IG135)

    Plasmid

    #99626

    Purpose

    To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    PGK-Neo
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

    Plasmid

    #231206

    Purpose

    Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
    

    Expression

    Mammalian

    Mutation

    L147I, R148H, I203V, L290V

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV8)

    Viral Prep

    #154868-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ulrik Gether

    Promoter

    Human Synapsin-1

    Tags

    mCherry (Flp-dependent)

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid

    #220612

    Purpose

    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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