We narrowed to 12,546 results for: NUC

1. NET33 pEGFP-N2 (597)

   Plasmid

   #61989

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET33 (SCARA5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7-RelA

   Plasmid

   #21984

   Depositor

   Warner Greene

   Article

   Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

   Insert

   NFkB p65 subunit (RELA Human)
   

   Use

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   For more details for this expression construct,…

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-C1-KSENB-Nup133

   Plasmid

   #87340

   Purpose

   To express human Nup133 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP133 (NUP133 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deletion of Nup133 AA1-7

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-SaCas9

   Plasmid

   #178901

   Purpose

   SaCas9 (Cas9 from Staphylococcus aureus)

   Depositor

   Hendrik Milting

   Article

   Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

   Insert

   SaCas9
   

   Use

   Tags

   His6-Tag, bipartite nuclear localization signal f…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-AgdNK

   Plasmid

   #203226

   Purpose

   For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. iSpyMac

   Plasmid

   #155014

   Purpose

   Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

   Insert

   iSpyMac
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PID grafted onto SpyCas9 N-terminal domain, R221K…

   Promoter

   EF1ɑ

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRFP-NLS

   Plasmid

   #105296

   Purpose

   To visualize nuclear dynamics in living cells

   Depositor

   Kenneth Yamada

   Article

   Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   aa encoding 172-192

   Promoter

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. WN10151

   Plasmid

   #80441

   Purpose

   Expresses human codon-optimized dead (832A) LbCpf1

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Insert

   dead (D832A) LbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDN34

   Plasmid

   #61343

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS2 and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1-Nup35-mut

    Plasmid

    #87341

    Purpose

    To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP35 (NUP35 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQCXIZ-turq-3xFLAG-TREX1 D18N

    Plasmid

    #164233

    Purpose

    retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and mturquoise

    Expression

    Mammalian

    Mutation

    D18N ->nuclease deficient

    Promoter

    CMV

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTriEx-mCherry::LANS4

    Plasmid

    #60785

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS4
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046785

    Plasmid

    #145684

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. BA31_pU.CAG.SaCas9-D10A.rBGpA

    Plasmid

    #199251

    Purpose

    Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SaCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTXB1-EXO1b

    Plasmid

    #68267

    Purpose

    Bacterial expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    T7

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAcrVIB1

    Plasmid

    #184566

    Purpose

    Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSFFV-dNme2Cas9

    Plasmid

    #193190

    Purpose

    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    dNme2Cas9
    

    Use

    Tags

    NLS and NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    H588A, D16A

    Promoter

    SFFV

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB22

    Plasmid

    #61342

    Purpose

    Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-cMyc1A NLS and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS315e_pGal-dCas9-PmCDA1

    Plasmid

    #79615

    Purpose

    Expresses dCas9-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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