We narrowed to 11,264 results for: Vars

1. PTPRA_deltaD1-GFP fusion

   Plasmid

   #139640

   Purpose

   GFP-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   PTPRA_deltaD1 (PTPRA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   D1 domain deletion

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. RET_Y981F-SHC

   Plasmid

   #139621

   Purpose

   StrepIII-HA-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   RET_Y981F (RET Human)
   

   Use

   Tags

   StrepIII-HA

   Expression

   Mammalian

   Mutation

   Y981F

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. gamma-tubulin (334-451)

   Plasmid

   #104436

   Purpose

   expressing a fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

   Insert

   gamma-tubulin fragment 334 to 451 (TUBG1 Human)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   deleted a.a. 1 to 333

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD2038

   Plasmid

   #101378

   Purpose

   Name: pBluescript-ΔRRE. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE.

   Depositor

   Dirk Dittmer

   Article

   Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

   Insert

   Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Blue Heron Biotechnology Inc. bidirectional mutan…

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC_N_KLF6

   Plasmid

   #167772

   Purpose

   Expresses KLF6 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   KLF6 (KLF6 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM41_KPNB1_HEAT

   Plasmid

   #178494

   Purpose

   Bacterial expression of human domain with His-tag and MBP tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   KPNB1_HEAT (KPNB1 Human, Synthetic)
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ERG_pet28a

   Plasmid

   #131642

   Purpose

   Bacterial expression of ETS gene ERG

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ERG (ERG Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES2_EGFP_KCNQ1

   Plasmid

   #173161

   Purpose

   For mammalian expression of KCNQ1 and creating variants

   Depositor

   Al George

   Article

   Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

   Insert

   KCNQ1 (KCNQ1 Human)
   

   Use

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-B-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207377

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ETS1_pet28a

    Plasmid

    #131663

    Purpose

    Bacterial expression of ETS gene ETS1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETS1 (ETS1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS003-lenti-CMV-GCaMPer

    Plasmid

    #65227

    Purpose

    ER calcium sensor for lentivirus production

    Depositor

    Brandon Harvey, Adam Cohen

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    circularly permuted GFP

    Expression

    Mammalian

    Mutation

    GCaMP3 (D324G, D360G, D397G, D435G)

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. ETV4_pet28a

    Plasmid

    #131645

    Purpose

    Bacterial expression of ETS gene ETV4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLI1_pet28a

    Plasmid

    #131641

    Purpose

    Bacterial expression of ETS gene FLI1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2FE-ABE8e-SpRY

    Plasmid

    #213008

    Purpose

    A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-shRNA

    Plasmid

    #82697

    Purpose

    For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    hPGK, U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS SARS-CoV-2 XBB Spike

    Plasmid

    #195287

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 XBB Spike

    Depositor

    Marceline Côté

    Insert

    SARS-CoV-2 XBB Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24-27…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    Feb. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218866

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218862

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SiriusGFP

    Plasmid

    #123201

    Purpose

    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

    Insert

    SiriusGFP
    

    Use

    Mouse Targeting

    Tags

    N/A

    Expression

    Bacterial and Mammalian

    Mutation

    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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