168,256 results

1. pDMyc-TNPO1

   Plasmid

   #86081

   Purpose

   A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Transportin-1 (TNPO1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ132B

   Plasmid

   #69282

   Purpose

   Golden Gate entry vector; 2nd gRNA under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH2B-miRFP680-N1

   Plasmid

   #197229

   Purpose

   Expresses the fusion protein of H2B-miRFP680 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   H2B-miRFP680
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

   Plasmid

   #236368

   Purpose

   Overexpression of HyperdLbCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   HyperdLbCas12a
   

   Use

   CRISPR and Lentiviral

   Mutation

   D156R, D235R, E292R, D350R

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSyn.iGluSnFR3.v857.PDGFR

   Plasmid

   #178329

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Has Service

   AAV1

   Insert

   pAAV hSyn iGluSnFR3 v857.PDGFR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and PDGFR TM Domain

   Expression

   Mammalian

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEVOL-NBK-1

   Plasmid

   #207639

   Purpose

   A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
   

   Expression

   Bacterial

   Mutation

   The evolved synthetase NBK-1 contains mutations Y…

   Promoter

   araBAD

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENN226_CRISPRcharm

   Plasmid

   #220834

   Purpose

   CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EGFP-C1-centrin-1

   Plasmid

   #73331

   Purpose

   Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag

   Depositor

   Manuel Thery

   Insert

   centrin-1 (CETN1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-VSVP

   Plasmid

   #64088

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

   Depositor

   Ian Wickersham

   Insert

   VSV phosphoprotein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiCas9

    Plasmid

    #162758

    Purpose

    express miCas9

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    Contains Brex27 sequence range is 4958-5065

    Promoter

    CMV

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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