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Plasmid#124400PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.DepositorInsertGal4-TET1-878-911 wildtype (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptet1
Plasmid#218367PurposeEmpty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3'UTR-Tetra-com-vector
Plasmid#132553PurposeExpresses spCas9 and sgRNA scaffold with com aptamer replacing the TetraloopDepositorInsertsCas9
sgRNA scaffold with com replacing the Tetraloop
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TetOne-Puro-p62 deltaUBA
Plasmid#204550PurposeExpresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cellsDepositorInsertp62 (SQSTM1 Human)
UseRetroviralTagsExpressionMammalianMutationdeltaUBAPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-KLF4-P2A-mCherry
Plasmid#203855PurposeTet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertKLF4 (KLF4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1#131-Tet-pLKO-puro
Plasmid#107400PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-MAFB-P2A-mCherry
Plasmid#203866PurposeTet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertMAFB (MAFB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-iCAM-1-Tether
Plasmid#205196PurposeThis vector encodes of the synCAM Tether (iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM iCAM1 Tether and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PLX306 iCre tetO NICD rtTA
Plasmid#158049Purposelenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domainDepositorInsertmouse Notch Intra Cellular Domain
UseTagsV5ExpressionMammalianMutationPromotertetO promoterAvailable sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-PB-PGK-tetR-KRAB-2A-Puro
Plasmid#129536PurposePiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.DepositorInsertPB-PGK-tetR-KRAB-2A-Puro
UseUnspecifiedTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX-tetO-Pcyc1-3xFLAG
Plasmid#41020DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U1-70K-fSNAPc
Plasmid#106987Purposeopen reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertU1-70K (SNRNP70 Human)
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-ID2-P2A-mCherry
Plasmid#203865PurposeTet-inducible lentiviral plasmid expressing ID2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertID2 (ID2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-LOV
Plasmid#22033PurposeMammalian expression of mVenus tagged LOV2 domainDepositorInsertmVenus-LOV
UseRetroviralTagsExpressionMammalianMutationInsert length based on Venus (720bp) + LOV (450bp)PromoterAvailable sinceSept. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1 #122-Tet-pLKO-puro
Plasmid#107401PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-TRPS1-P2A-mCherry
Plasmid#203851PurposeTet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertTRPS1 (TRPS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-iRFP
Plasmid#176028Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720DepositorInsertstetR
iRFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX-tetO-Pcyc1-3xFLAG
Plasmid#41021DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
Plasmid#129537PurposePiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.DepositorInserttetR-3xFlag-HA-2A-Puro
UseSynthetic BiologyTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1
Plasmid#22008PurposeMammalian expression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
UseRetroviralTagsmVenusExpressionMammalianMutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only