We narrowed to 15,977 results for: LEA

1. SDCBPA-c011

   Plasmid

   #175483

   Purpose

   Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), A106-F275.

   Depositor

   Opher Gileadi

   Insert

   SDCBP (SDCBP Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TBXTA-c025

   Plasmid

   #139762

   Purpose

   Protein expression/GST pull-down

   Depositor

   Opher Gileadi

   Insert

   TBXT, Full-length, G177D (TBXT Human)
   

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   G177D; contains only amino acids S2- M435

   Promoter

   T7

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TBXTA-c023

   Plasmid

   #139760

   Purpose

   Protein expression/GST pull-down

   Depositor

   Opher Gileadi

   Insert

   TBXT, DNA-binding domain, WT (TBXT Human)
   

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   Contains only amino acids E41-D225

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TBXTA-c022

   Plasmid

   #139759

   Purpose

   Protein expression/crystallography

   Depositor

   Opher Gileadi

   Insert

   TBXT, DNA-binding domain, G177D (TBXT Human)
   

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   G177D; contains only amino acids E41-A214

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease

   Plasmid Kit

   #1000000087

   Purpose

   Tol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disorders

   Depositor

   Nicholas Cole

   Available Since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-mCherry

   Plasmid

   #128744

   Purpose

   Expresses mCherry in mammalian cells

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GST-MNase

   Plasmid

   #136291

   Purpose

   Expresses Micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   MNase
   

   Expression

   Bacterial

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PRDX1A-c001

   Plasmid

   #175473

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX1 (PRDX1 Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOpen-RNAse A

   Plasmid

   #165565

   Purpose

   Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   RNAse A
   

   Use

   Synthetic Biology

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGEX6P1-DEST

    Plasmid

    #119749

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHMGWA-Pa14Csy4H29A

    Plasmid

    #41092

    Depositor

    Jennifer Doudna

    Article

    Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

    Insert

    Csy4
    

    Tags

    His6-MBP

    Mutation

    His29Ala mmutation to abolish ribonuclease activi…

    Promoter

    T7

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pGEX6P1-N-HA

    Plasmid

    #119756

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Tags

    GST and HA

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CNN3A-c002

    Plasmid

    #175456

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-Y329.

    Depositor

    Opher Gileadi

    Insert

    CNN3 (CNN3 Human)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TBXTA-c028

    Plasmid

    #139765

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, Full-length, WT (TBXT Human)
    

    Tags

    Avi-tag (Biotin) and His6-GST-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids S2- M435

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TBXTA-c043

    Plasmid

    #139766

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, WT (TBXT Human)
    

    Tags

    Avi-tag (Biotin) and His6-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids E41- D225

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF6V5:eGFP-CAAX-2A-mCherry

    Plasmid

    #26901

    Depositor

    Steven Leach

    Article

    Provost et al Genesis. 2007 Oct . 45(10):625-9.

    Insert

    eGFP-CAAX-PTVI-2A-mCherry
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

17. TBXTA-c027

    Plasmid

    #139764

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, Full-length, G177D (TBXT Human)
    

    Tags

    Avi-tag (Biotin) and His6-GST-TEV

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids S2- M435

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TBXTA-c044

    Plasmid

    #139767

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Tags

    Avi-tag (Biotin) and His6-TEV

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41- D225

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCYP119

    Plasmid

    #50771

    Purpose

    expresses CYP 119 in e. coli

    Depositor

    Stephen Sligar

    Article

    McLean et al Biochem Biophys Res Commun. 1998 Nov 9;2

    Insert

    CYP119
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Ec FkP-dnaN(3E's)-pRSFDuet

    Plasmid

    #238344

    Purpose

    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli

    Depositor

    Michael O'Donnell

    Insert

    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
    

    Tags

    3X FLAG+PKA kinase motif+PreScission Protease cle…

    Expression

    Bacterial

    Mutation

    R96E; R103E; R105E

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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