We narrowed to 3,816 results for: golden gate

1. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

   Plasmid

   #219711

   Purpose

   Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.

   Depositor

   Jennifer Nemhauser

   Insert

   GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
   

   Use

   Synthetic Biology

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MUT_S1-N

   Plasmid

   #218398

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MUT_N-H1

   Plasmid

   #218397

   Purpose

   3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK7-g7g8-K8

   Plasmid

   #218220

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK3-g3g4-K4

   Plasmid

   #218218

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK5-g5g6-K6

   Plasmid

   #218219

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP4_6IYE

   Plasmid

   #234237

   Purpose

   Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. MUT_S1-H1

   Plasmid

   #218394

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAIP4_2WWE

   Plasmid

   #234210

   Purpose

   Heterologous protein expression of PI3K-C2-gamma in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2WWE (PIK3C2G Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

    Plasmid

    #219687

    Purpose

    Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPtGG-1

    Plasmid

    #89990

    Purpose

    pBR322-based entry vector for Gateway cloning

    Depositor

    Philip Weyman

    Article

    Bielinski et al PeerJ Preprints. 2017 5:e2907v1

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Available Since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMV_AA100

    Plasmid

    #245322

    Purpose

    Cas9 CRISPRko all-in-one positive control; targets CD55

    Depositor

    John Doench, David Root

    Insert

    Cas9, sgRNA CD55 (CD55 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMV_AA104

    Plasmid

    #245326

    Purpose

    Cas9 CRISPRa positive control guide; targets CD4

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD4 (CD4 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-Kan-HC-L0-TaBSS1-3D

    Plasmid

    #242008

    Purpose

    L0 3U* Golden Gate part of 3' UTR and downstream regulatory regions of Triticum aestivum MANY-NODED DWARF 1 (MND1) B-subgenome homoeolog (TraesCS7B02G413900)

    Depositor

    Cristobal Uauy

    Insert

    TaBSS1-3D
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAttPF3Hyg

    Plasmid

    #244929

    Purpose

    attP Hyg Resistant plasmid for RMI targeting an attB site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAttBFGNeo2

    Plasmid

    #244928

    Purpose

    attB Neo Resistant mNeonGreen marked plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion vector

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAttBFGNeo

    Plasmid

    #244925

    Purpose

    attB Neo Resistant mNeonGreen marked plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAttBFNeo

    Plasmid

    #244923

    Purpose

    attB Neo Resistant plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAttBFNeo2

    Plasmid

    #244924

    Purpose

    attB Neo Resistant plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAttPF3Neo

    Plasmid

    #244930

    Purpose

    attP Neo Resistant plasmid for RMI targeting an attB site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge