We narrowed to 2,607 results for: ubiquitin

1. pGEX-6P1-Ataxin3Q22-C14A

   Plasmid

   #22113

   Depositor

   Henry Paulson

   Article

   Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C14A

   Promoter

   Available since

   Sept. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

2. PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)

   Plasmid

   #119319

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP III (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 1-84

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT-ubb-NLS-BirA-2a-mCherry

   Plasmid

   #79886

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3xFLAG-CMV-RNF8-N-terminus(1-140)

   Plasmid

   #64675

   Purpose

   expresses only N terminus of RNF8, amino acids 1 to 140

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   Ring finger protein 8 (RNF8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   only N terminus amino acids 1-140

   Promoter

   CMV

   Available since

   May 20, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMT-ubb-Avi-Cerulean-RanGAP

   Plasmid

   #79881

   Purpose

   Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
   

   Use

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-RNF8-Middle-part

   Plasmid

   #64672

   Purpose

   Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   ring finger protein 8 (RNF8 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Containing only middle part of RNF8 amino acids 1…

   Promoter

   CMV

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pOPINK-OTUD6A (OTU, aa 128-288)

   Plasmid

   #61417

   Purpose

   Expresses human OTUD6A (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6A (OTUD6A Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-127.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBacPak-HA-E(y)2

   Plasmid

   #52288

   Purpose

   Expresses Drosophila E(y)2

   Depositor

   Jerry Workman

   Article

   Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

   Insert

   E(y)2 (e(y)2 Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCT-CMV-UBE3BΔHECT-copGFP-Puro

   Plasmid

   #107343

   Purpose

   Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B(ΔHECT)-copGFP (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   copGFP

   Expression

   Mammalian

   Mutation

   deletion of HECT domain (757-end)

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pEGFP-WRAP53beta-DC-mutant-G435R

    Plasmid

    #64684

    Purpose

    expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutation at G435R

    Promoter

    CMV

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEGFP-WRAP53beta-DC-mutant-R398W

    Plasmid

    #64683

    Purpose

    expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutation at R398W

    Promoter

    CMV

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pOPINK-OTUD6B (OTU, aa 133-293)

    Plasmid

    #61419

    Purpose

    Expresses human OTUD6B (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-132.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p7055 pHAGE-P-CMVt-N-HA-GAW-COPZ1

    Plasmid

    #100145

    Purpose

    Expresses COPZ1

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    COPZ1 (COPZ1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-WRAP53beta-DC-mutant-F164L

    Plasmid

    #64681

    Purpose

    expresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutation at F164L

    Promoter

    CMV

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pEGFP-WRAP53beta-DC-mutant-H376Y

    Plasmid

    #64682

    Purpose

    expresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutation at H376Y

    Promoter

    CMV

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

16. GP804

    Plasmid

    #164548

    Purpose

    Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    All sixteen Lysines in cytoplasmic C-terminal tai…

    Promoter

    GgCryD1

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOPINK-OTUD2 (OTU, aa 132-314)

    Plasmid

    #61410

    Purpose

    Expresses human OTUD2 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-131 and aa 315-348.

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5FRT/TO PP1gamma-2*Strep

    Plasmid

    #113506

    Purpose

    Expresses dual Strep-tagged PP1gamma in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    PP1gamma (PPP1CC Human)
    

    Use

    Tags

    Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/GS-DJ1-E64D-V5

    Plasmid

    #29344

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His6 and V5 epitope

    Expression

    Mammalian

    Mutation

    Glu64Asp

    Promoter

    Available since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-6P1-Ataxin3Q22-UIM*

    Plasmid

    #22114

    Depositor

    Henry Paulson

    Article

    Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    UIM 1, 2, 3 SA-DG

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only