We narrowed to 2,690 results for: BFP

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VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

Plasmid

#242407

Purpose

Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-moxBFP

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-Ns3h-UGI-T2A-mTagBFP2

Plasmid

#221223

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-Ns3h-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

Plasmid

#191146

Purpose

Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdnf.t1

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#164046

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mTagBFG-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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MADR pDonor TagBFP2-3XFlag (cyto) WPRE

Plasmid

#129421

Purpose

MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

Depositor

Joshua Breunig

Article

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

TagBFP2-3Flag

Use

Mosaic analysis for dual recombinase-mediated cas…

Tags

3X Flag epitope tag

Promoter

none (3X polyA to mitigate episomal expression)

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#213480

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA-directcapture

Use

AAV

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

Plasmid

#180587

Purpose

FdCd

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Cre

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

Plasmid

#176027

Purpose

construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

rtTA3
Luc2
mtagBFP

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pubc-FRB-BFP-IRES-FKBP-HaloTag_tdMCP-NLS-HA (RIDR negative control)

Plasmid

#224133

Purpose

RIDR negative control.

Depositor

Bin Wu

Article

Blake et al Nat Commun. 2024 Mar 28;15(1):2720. doi:

Insert

pubc-FRB-tagBFP-IRES-FKBP-HaloTag_tdMCP-NLS-HA

Tags

FKBP-HaloTag-tdMCP-NLS-HA and FRB-tagBFP

Expression

Mammalian

Promoter

ubc

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)

Plasmid

#107860

Purpose

To quickly insert CTD mutants into TDP43 splicing reporter

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of CTD in TDP43 splicing reporter, inser…

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only
pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)

Plasmid

#107855

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G368W and W385G in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187951

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

Plasmid

#184952

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mTagBFP2

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV GST mTagBFP

Plasmid

#206259

Purpose

ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

GTS mTagBFP

Use

Multimate/gateway entr 4

Tags

BFGT1 (Golgi localisation peptide)

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pscAAV-CBh-tagBFP2-WPRE3-enSV40pA

Plasmid

#194246

Purpose

Expresses tagBFP2

Depositor

Michael J Fox Foundation MJFF

Insert

tagBFP2

Use

AAV

Promoter

CBh

Available Since

May 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA27-pHR-MiniCMV-TriTag (mTagBFP)

Plasmid

#199458

Purpose

Expression of TriTag (mTagBFP) in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TriTag

Use

Lentiviral

Expression

Mammalian

Promoter

MiniCMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

Plasmid

#178868

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FRT-EBFP-tTAn

Plasmid

#172127

Purpose

Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT7-A10)-PGKpuro2ABFP-W

Plasmid

#159288

Purpose

Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. A10)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT7 (ID. A10) (KAT7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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