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BASIC_SEVA_17_pKD46.10

Plasmid

#178917

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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OmpT

Plasmid

#213137

Purpose

OmpT_native membrane expression

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

OmpT

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDJSVT91

Plasmid

#109305

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

SufI 1-30, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-TTP(C147R)-GG-PUF

Plasmid

#52138

Purpose

Receiving vector for PUM-HD assembly and expression in mammalian cells

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Tags

(GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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MCS Hras FI

Plasmid

#90413

Purpose

A control of MCS+ that associated with cell plasma membrane by two hydrophobic interaction at N and C terminus of the FRET pair.

Depositor

Katharina Gaus

Article

Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

Insert

Lck10+ Venus+mcherry + H-ras C terminus

Expression

Mammalian

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSM03

Plasmid

#139043

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

dendra2 (includes STOP codon)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Venus-KRasCT

Plasmid

#99016

Purpose

Membrane marker

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Venus KRasCT

Expression

Mammalian

Promoter

CM

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2845

Plasmid

#87763

Purpose

Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pnmr-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pnmr-1

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2838

Plasmid

#87759

Purpose

Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmyo-3-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pmyo-3

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-BoxB-A114-N40

Plasmid

#50562

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-BoxB-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCZGY2842

Plasmid

#87762

Purpose

Punc-25-PH::miniSOG, expression of membrane targeted PH-miniSOG in GABAergic motor neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-25-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Punc-25

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_C_DUX4

Plasmid

#187799

Purpose

MAC-tagged gene expression of human DUX4

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4 (DUX4 Human)

Expression

Mammalian

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC

Plasmid

#170772

Purpose

test plasmid for Golden Gate assembly, expresses lacI repressor

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

lacI

Promoter

lacI promoter

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMXLK_aFb

Plasmid

#114230

Purpose

POC12368, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046310

Plasmid

#149022

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAPTW

Plasmid

#205695

Purpose

shRNAmir backbone for RNA interference, with mTagBFP2 reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCZGY2840

Plasmid

#87764

Purpose

Pmec-4-PH::miniSOG, expression of membrane targeted PH-miniSOG in mechanosensory neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pmec-4

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSDH57

Plasmid

#139047

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mScarlet (no STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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BiDi-[mp-mCherry2]mp-mEGFP

Plasmid

#182871

Purpose

Bi-directional expression vector of monomers with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mCherry2, myr-palm-mEGFP

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSM12

Plasmid

#139035

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

dendra2 (no STOP codon)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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