We narrowed to 2,467 results for: GCG

1. pLenti-v2-ALDH1A3gRNA2

   Plasmid

   #189747

   Purpose

   The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   Cas9, ALDH1A3 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPbuCas13b-gRNA-1

   Plasmid

   #184567

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

   Plasmid

   #168251

   Purpose

   "label active Rac under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   PBD GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

   Plasmid

   #168254

   Purpose

   "label stable F-actin under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Utrophin-GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mU6-sgCebpa_v2-hu6-sgCebpb_v2

   Plasmid

   #177252

   Purpose

   Expresses Cebpa_v2 (mU6), Cebpb_v2(hU6) gRNAs and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

   Insert

   sgCebpa_v2/sgCebpb_v2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6/hU6

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mU6-sgCebpa_v2-hu6-sgCebpd_v2

   Plasmid

   #177254

   Purpose

   Expresses Cebpa_v2 (mU6), Cebpd_v2(hU6) gRNAs and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

   Insert

   sgCebpa_v2/sgCebpd_v2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6/hU6

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. hU6-sgNeo1 (opti)

   Plasmid

   #177240

   Purpose

   Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

   Depositor

   Monte Winslow

   Article

   Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

   Insert

   sgNeo1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. hu6-sgCebpa_v2 (opti)

   Plasmid

   #177246

   Purpose

   Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

   Depositor

   Monte Winslow

   Article

   Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

   Insert

   sgCebpa_2nd
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_BRD003 Ch2-2

   Plasmid

   #184486

   Purpose

   Lentivirus gRNA targeting Ch2-2 (control)

   Depositor

   Peter Tsvetkov, Todd Golub

   Article

   Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

   Insert

   Ch2-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-HypaCas9-mR2-CfANLN_sgRNA

    Plasmid

    #183879

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    Canis familiaris ANLN sgRNA spacer
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-HypaCas9-H2BC11_sgRNA

    Plasmid

    #183883

    Purpose

    pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 sgRNA spacer (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-MYH10_sgRNA

    Plasmid

    #183887

    Purpose

    pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 sgRNA spacer (MYH10 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-HypaCas9-mR2-H2BC11_sgRNA

    Plasmid

    #183881

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 sgRNA spacer (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459-HypaCas9-mR2-MYH10_sgRNA

    Plasmid

    #183886

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 sgRNA spacer (MYH10 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.U6gT28.4.hSyn.H2B.RFP

    Plasmid

    #170387

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiGuide-PolB1-puro

    Plasmid

    #177146

    Purpose

    Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolBgRNA1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRv1_COX4

    Plasmid

    #177983

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting COX4

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting COX4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

    Plasmid

    #173212

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV.U6gT28.4.EFS-NS.H2B-RFP

    Plasmid

    #170365

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS-NS

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Grin1 gRNA#2

    Plasmid

    #169790

    Purpose

    Cas9-mediated knockout of Grin1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge