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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,144 results for: LEA

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Showing: 1441 - 1460 of 16144 results

  2. TBXTA-c021

    Plasmid
    #139758
    Purpose
    Protein expression/crystallography (PDB:5QT0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-N211
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139758-plasmid-map-sequence-id-296541

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  4. SDCBPA-c010

    Plasmid
    #175482
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175482-plasmid-map-sequence-id-353168

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  5. TBXTA-c020

    Plasmid
    #139757
    Purpose
    For protein expression/crystallography (PDB 5QS0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-N211
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139757-plasmid-map-sequence-id-296518

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  6. pmCer-RPA32

    Plasmid
    #211385
    Purpose
    mammalian expression of human RPA32 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211385-plasmid-map-sequence-id-420500

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  7. SDCBPA-c021

    Plasmid
    #175485
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), G111-F275.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175485-plasmid-map-sequence-id-353207

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  8. SDCBPA-c011

    Plasmid
    #175483
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), A106-F275.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175483-plasmid-map-sequence-id-353170

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  9. TBXTA-c025

    Plasmid
    #139762
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, G177D (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids S2- M435
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139762-plasmid-map-sequence-id-296575

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  10. TBXTA-c023

    Plasmid
    #139760
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-D225
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139760-plasmid-map-sequence-id-296543

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  11. TBXTA-c022

    Plasmid
    #139759
    Purpose
    Protein expression/crystallography
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-A214
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139759-plasmid-map-sequence-id-296542

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  12. pGEX6P1-DEST

    Plasmid
    #119749
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119749-plasmid-map-sequence-id-233993

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  15. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  16. PRDX1A-c001

    Plasmid
    #175473
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX1 (PRDX1 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    175473-plasmid-map-sequence-id-353180

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  17. pGEX6P1-N-HA

    Plasmid
    #119756
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and HA
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119756-plasmid-map-sequence-id-233665

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  19. CNN3A-c002

    Plasmid
    #175456
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-Y329.
    Depositor
    Opher Gileadi
    Insert
    CNN3 (CNN3 Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175456-plasmid-map-sequence-id-353174

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  20. TBXTA-c028

    Plasmid
    #139765
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, WT (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids S2- M435
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139765-plasmid-map-sequence-id-296580

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Showing: 1441 - 1460 of 16144 results