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Addgene

We narrowed to 15,783 results for: LEA

Showing: 1441 - 1460 of 15783 results
  1. TBXTA-c044

    Plasmid
    #139767
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41- D225
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. Ec FkP-dnaN(3E's)-pRSFDuet

    Plasmid
    #238344
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96E; R103E; R105E
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. Ec FkP-dnaN(3A's)-pRSFDuet

    Plasmid
    #238343
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96A; R103A; R105A
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. Ec FkP-dnaN(3S's)-pRSFDuet

    Plasmid
    #238345
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96S; R103S; R105S) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96S; R103S; R105S
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. CNN3A-c007

    Plasmid
    #175457
    Purpose
    Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.
    Depositor
    Insert
    CNN3 (CNN3 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. TBXTA-c024

    Plasmid
    #139761
    Purpose
    Protein expression/GST pull-down
    Depositor
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-D225
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pGEX6P1-N-His

    Plasmid
    #119755
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.
    Type
    Empty backbone
    Use
    Tags
    10xHis and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pGEX6P1-N-FLAG

    Plasmid
    #119759
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.
    Type
    Empty backbone
    Use
    Tags
    FLAG and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pGEX6P1-N-Strep2

    Plasmid
    #119758
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.
    Type
    Empty backbone
    Use
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. EPB41L3A-c000

    Plasmid
    #175458
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-D1087.
    Depositor
    Insert
    EPB41L3 (EPB41L3 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pGEX6P1-N-Myc

    Plasmid
    #119757
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.
    Type
    Empty backbone
    Use
    Tags
    GST and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pGEX6P1-DEST-HA

    Plasmid
    #119751
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.
    Type
    Empty backbone
    Use
    Tags
    GST and HA
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pGBW-m4133370

    Plasmid
    #152013
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;CBP
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1441 - 1460 of 15783 results