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pMRE163

Plasmid

#118511

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTmpSm

Plasmid

#176228

Purpose

A template plasmid for amplification of the cI-hok-strA cassette

Depositor

Sergey Sineoky

Publication

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

cI of phage lambda, hok of the R1 plasmid, strA

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFG036

Plasmid

#137996

Purpose

cI repressor expression plasmid

Depositor

Sébastien Rodrigue

Publication

Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

Insert

cI

Use

Synthetic Biology

Expression

Bacterial

Mutation

Thermosensitive

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE167

Plasmid

#118515

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPI459

Plasmid

#113196

Purpose

extrachromosomal expression vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector, extra…

Tags

mScarlet

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Expression

Worm

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pQCascade-IS186

Plasmid

#140627

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186

Use

CRISPR; Transposon

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS-TarHom-Tol2-kanaR-cryaa-YFP

Plasmid

#74154

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Publication

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-YFP

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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Frt-hspB4

Plasmid

#63095

Purpose

Expression of HSPB4 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Publication

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb4 (CRYAA Human)

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pICH78133

Plasmid

#50292

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Publication

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pPI278

Plasmid

#113227

Purpose

extrachromosomal expression vector, expression of H2B-GFP

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

H2B

Use

Dictyostelium discoideum expression vector, extra…

Tags

GFP

Promoter

act15

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Publication

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Expression

Insect

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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WDR4_pCSdest

Plasmid

#53953

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

WDR4 (WDR4 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pMpGWB230

Plasmid

#68621

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

tdTomato

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pBT225_(pCA-tdT3Myc)

Plasmid

#36873

Depositor

Liqun Luo

Publication

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Tags

3 Myc tags

Expression

Mammalian

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA-NC1

Plasmid

#248557

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA-NC1 TD#277

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-130

Plasmid

#118516

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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