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We narrowed to 6,562 results for: nls

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Showing: 1441 - 1460 of 6562 results

  1. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    139094-plasmid-map-sequence-id-273891

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  2. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid
    #192552
    Purpose
    Expresses nuclear localized tdTomato under CAG promoter
    Depositor
    Guoping Feng
    Has service
    AAV9 and AAV PHP.eB
    Insert
    tdTomato
    Use
    AAV
    Tags
    NLS
    Mutation
    deleted E273 and G274 compared to FPbase ID: PGG5S
    Promoter
    CAG
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192552-plasmid-map-sequence-id-403193

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  8. pNB693

    Plasmid
    #60145
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60145-plasmid-map-sequence-id-96228

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  9. pNB689

    Plasmid
    #60133
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60133-plasmid-map-sequence-id-96189

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  10. pNB756

    Plasmid
    #60149
    Purpose
    Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    LEU1pr
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60149-plasmid-map-sequence-id-96311

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  11. pNB752

    Plasmid
    #60155
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    ADE17pr
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60155-plasmid-map-sequence-id-96323

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  12. pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS

    Plasmid
    #154223
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 11xQUAS+CP_pcbC
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154223-plasmid-map-sequence-id-306046

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  13. pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS

    Plasmid
    #154238
    Purpose
    Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    pgndA:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    pGndA (An11g02040 A. niger)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154238-plasmid-map-sequence-id-305980

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  14. enOgeuIscB with ωRNA-v2

    Plasmid
    #220939
    Purpose
    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing
    Depositor
    Weixin Tang
    Publication
    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/
    Inserts
    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220939-plasmid-map-sequence-id-443121

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  16. TC1464

    Plasmid
    #164881
    Purpose
    CMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1
    Depositor
    Tian Chi
    Publication
    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas13d-ADARdd
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164881-plasmid-map-sequence-id-325955

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  18. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Publication
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Promoter
    T7
    Available since
    Oct. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

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  20. NLS-tdTomato

    Plasmid
    #163443
    Purpose
    Bacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backbone
    Depositor
    Sanford Simon
    Insert
    tdTomato
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163443-plasmid-map-sequence-id-321654

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Showing: 1441 - 1460 of 6562 results