We narrowed to 43,126 results for: cha

1. pAW8ENdeI-cyEGFP-8XDSR

   Plasmid

   #49866

   Purpose

   for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   yEGFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAW8ENdeI-cyEGFP-4XDSR

   Plasmid

   #49864

   Purpose

   for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   yEGFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAW8ENdeI-c3HA-8XDSR

   Plasmid

   #49871

   Purpose

   for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   3XHA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAW8ENdeI-c3HA-4XDSR

   Plasmid

   #49869

   Purpose

   for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   3XHA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSUPER.retro.puro-THAP11-A

   Plasmid

   #36993

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Insert

   THAP11 shRNA (THAP11 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pSUPER.retro.puro-THAP11-C

   Plasmid

   #36994

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Insert

   THAP11 shRNA (THAP11 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pSUPER.retro.puro-THAP11-E

   Plasmid

   #36995

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Insert

   THAP11 shRNA (THAP11 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pET-sCS4T

   Plasmid

   #28249

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   single chain Cse4-Scm3-H4 with Thrombin site
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   A thrombin cutting site between Cse4 and Scm3

   Promoter

   Available since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pET-sCS4T+N

   Plasmid

   #28255

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   sCS4T+N
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   single chain CSE4-SCM3-H4 with Thrombin site, SCM…

   Promoter

   Available since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pET-sC4T

    Plasmid

    #28251

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain Cse4-H4 with Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between Cse4 and H4

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

11. piHGpd/T22I

    Plasmid

    #1110

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with threonine 22 changed to isoleucine; re…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. piHGpd/G81S

    Plasmid

    #1111

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with glycine 81 changed to serine; regulate…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. piHGpd/G313S

    Plasmid

    #1114

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with glycine 313 changed to serine; regulat…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

14. piHGpd/E381K

    Plasmid

    #1115

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with glutamic acid 381 changed to lysine; r…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

15. piHGpd/A587T

    Plasmid

    #1116

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with alanine 587 changed to threonine; regu…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

16. piHGpd/A41V

    Plasmid

    #1117

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with alanine 41 changed to valine; regulate…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pILGFP4G

    Plasmid

    #104502

    Purpose

    Used to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    ScPGM2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pILGFP4K

    Plasmid

    #104506

    Purpose

    Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SpGAL1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pILGFP4O

    Plasmid

    #104510

    Purpose

    Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SpdGAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pILGFP4P

    Plasmid

    #104511

    Purpose

    Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SptGAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. NaVSp1 FL DeltaCTD pIRES

    Plasmid

    #128077

    Purpose

    Mammalian cell expression vector for NaVSp1 bacterial sodium channel. Residues 1-224.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVSp1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NaVAe1 FL DeltaCTD pIRES

    Plasmid

    #128078

    Purpose

    Mammalian cell expression vector for NaVAe1 bacterial sodium channel. Residues 1-241.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NaVAe1 DeltaCC pIRES

    Plasmid

    #128086

    Purpose

    Mammalian cell expression vector for NaVAe1 1-264 chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUAP1

    Plasmid

    #63674

    Purpose

    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

    Depositor

    Nicola Patron

    Article

    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

    Insert

    RFP cloning selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLD-puro-MTS-EGFP

    Plasmid

    #190270

    Purpose

    Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

    Depositor

    Christian Münch

    Article

    Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

    Insert

    MTS-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL3-Basic-IRES

    Plasmid

    #64784

    Purpose

    Luciferase reporter vector

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tTRE, hPGK

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFLP-hyg

    Plasmid

    #87831

    Purpose

    Expresses FLP recombinase with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pACBSR-hyg

    Plasmid

    #87830

    Purpose

    Expresses lambda-red system with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    bla

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ZJOM90

    Plasmid

    #133647

    Purpose

    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLVX-puro_GCaMP6s

    Plasmid

    #164589

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTW073

    Plasmid

    #64754

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEx-deletion-erm

    Plasmid

    #172213

    Purpose

    For allelic exchange in erythromycin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Allelic exchange in prevotella copri

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEx-deletion-tet

    Plasmid

    #172214

    Purpose

    For allelic exchange in tetracyclin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Allelic exchange in prevotella copri

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDNR-gRNA

    Plasmid

    #206990

    Purpose

    A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Insert

    Donor plasmid-targeting SaCas9-gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HcKCR1_mCherry_pcDNA3.1

    Plasmid

    #177336

    Purpose

    Expresses KCR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid

    #186310

    Purpose

    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg61-PDGFR
    eGFP
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCR1307

    Plasmid

    #111133

    Purpose

    Expresses WDR48.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    WDR48 (WDR48 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMYCO1_SpdCas9_pmcDA1

    Plasmid

    #204615

    Purpose

    CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge