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pCAGGS-mCherry-C

Plasmid

#203326

Purpose

Express N-terminal mCherry-fused protein in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMZ4004

Plasmid

#204401

Purpose

A 3OHC14-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

mCinR
GFP

Use

Synthetic Biology

Promoter

PftsH and Synthetic promoter PCin0102D

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-FCU1

Plasmid

#165882

Purpose

Basic 5-fluorocytosine sensitivity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL12006

Plasmid

#50270

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5'UTR (Cassava Vein Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80005

Plasmid

#50322

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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rSytIV pIE

Plasmid

#12503

Depositor

Jane Sullivan

Article

Ting et al J Neurosci. 2006 Jan 11. 26(2):372-80.

Insert

Synaptotagmin IV (Syt4 Rat)

Tags

IRES EGFP

Expression

Mammalian

Available Since

Oct. 23, 2006

Availability

Academic Institutions and Nonprofits only

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pBS/U6 ezrin siRNA

Plasmid

#8945

Depositor

Philip Hinds

Article

Yang et al Mol Cell. 2003 May . 11(5):1163-76.

Insert

ezrin siRNA (EZR Human)

Expression

Mammalian

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-MKI

Plasmid

#65457

Purpose

photoactivatable myosin light-chain kinase inihibition

Depositor

Klaus Hahn

Article

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

MKI

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pTH-MYCN

Plasmid

#35416

Depositor

William A. Weiss

Article

Weiss et al EMBO J. 1997 Jun 2;16(11):2985-95.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN Human)

Expression

Mammalian

Promoter

Tryosine Hydroxlyase (TH)

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pET28C-tCorn

Plasmid

#124588

Purpose

Bacterial Expression of tRNA-Corn aptamer

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Bacterial

Promoter

lacI

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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mOrange pHis-//

Plasmid

#53304

Purpose

Empty vector to express N-terminal His-mOrange-tagged fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Type

Empty backbone

Tags

His and mOrange

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCJH027_SC101_pLAC_LbCas12a_Kan

Plasmid

#183074

Purpose

Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pICH71431

Plasmid

#50344

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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MLV Gag-CFP

Plasmid

#1812

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

CFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AcGFP-C

Plasmid

#203325

Purpose

Express N-terminal AcGFP-fused protein in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

AcGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-bsr

Plasmid

#165878

Purpose

Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5B-FLAG-SARA dSBD

Plasmid

#11737

Depositor

Jeff Wrana

Article

Tsukazaki et al Cell. 1998 Dec 11. 95(6):779-91.

Insert

SARA (ZFYVE9 Human)

Tags

Flag

Expression

Mammalian

Mutation

deltaSBD: lacks Smad2-binding domain (aa665-704)

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits
M2660 ura3::LYS2 Disruptor Converter

Plasmid

#51678

Purpose

Yeast marker swap plasmid for converting URA3 to LYS2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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