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Plasmid#169637PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2354_Tier3(PB)-Ruby_PuroR
Plasmid#169653PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPhCMV / PPGK / RPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2333_Tier3(Lenti_inverse)-YPet_Puro
Plasmid#169664PurposeTier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)DepositorInsertPRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPhCMV / RPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH663
Plasmid#169607PurposeTier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
ExpressionMammalianPromotertetO7-PCMVmin / PPGK / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH19-Tier1-PhCMV-p300core
Plasmid#169572PurposeTier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)DepositorAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2304-Tier1-Tactb-PPGK-NLuc_TB
Plasmid#169549PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertTactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
ExpressionMammalianPromoterPPGKAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1046-Tier1-PhCMV-4xCox8-mRuby2
Plasmid#169537PurposeTier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)DepositorInsertPCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Ala2
Plasmid#71326PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
ExpressionBacterialMutationD196A, L197RPromoterinsulated waaHp from E. coliAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA-xylA
Plasmid#158612PurposeLow phosphate inducible gRNA array to silence the xylA and udhA promotersDepositorInsertudhA-xylA guide array
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induct…Available SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2-xylA
Plasmid#158613PurposeLow phosphate inducible gRNA array to silence the xylA and gltA2 promotersDepositorInsertgltA2-xylA guide array
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induct…Available SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-zwf-xylA
Plasmid#158614PurposeLow phosphate inducible gRNA array to silence the xylA and zwf promotersDepositorInsertzwf-xylA guide array
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induct…Available SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-eGFP
Plasmid#126716Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
Tags6xHisExpressionYeastPromoterICL1Available SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Phhf2-PYC-Tadh1
Plasmid#127737PurposeYeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyExpressionYeastPromoterPhhf2Available SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ACS1-eGFP
Plasmid#126715Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterACS1Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ALD-eGFP
Plasmid#126714Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterALDAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-HXT1-eGFP
Plasmid#126719Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterHXT1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ADH3-eGFP
Plasmid#126713Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterADH3Available SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_lacO-cP-AOX1-sAR
Plasmid#126743Purposeyeast plasmid overexpressing CPR and sLovADepositorInsertslovA,cpr
Tags6xHisExpressionYeastAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPrps3AvailabilityAcademic Institutions and Nonprofits only