We narrowed to 11,225 results for: NSI;

1. pSB161 - pL2_pSB90_2x35S::GUS::tMAS

   Plasmid

   #123197

   Purpose

   binary plant vector for transient expression of GUS (with introns)

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::GUS::tMAS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBtPIPLC His

   Plasmid

   #173808

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Anne Gershenson, Mary F Roberts

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-ASAP2f

   Plasmid

   #183757

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   ASAP2f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX nsp1 R99A CoV2

   Plasmid

   #175515

   Purpose

   For protein expression of R99A nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   R99A nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO-BcRF1-2xStrep

   Plasmid

   #138436

   Purpose

   Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   BcRF1 (BcRF1 EBV (HHV-4))
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. b2-TS3

   Plasmid

   #103877

   Purpose

   full-length human b2 with tension sensor at b2_733

   Depositor

   Timothy Springer

   Article

   Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

   Insert

   full-length human b2 with tension sensor at b2_733 (ITGB2 Human)
   

   Use

   Tags

   tension sensor module

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1_jRCaMP1b

   Plasmid

   #183758

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   jRCaMP1b
   

   Use

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO-UL87-2xStrep

   Plasmid

   #138434

   Purpose

   Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   UL87 (UL87 HCMV (HHV-5))
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgCTRL-hygro

   Plasmid

   #199639

   Purpose

   sgRNA control; induces CAS9 cutting in an intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3.1 AT1AR-TSTS/4A

    Plasmid

    #45634

    Depositor

    Robert Lefkowitz

    Article

    Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

    Insert

    AT1AR-TSTS/A (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Thr332, Ser335, Thr336, and Ser338 all changed to…

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pMAL-c2X-ORF24-NTD

    Plasmid

    #138465

    Purpose

    Expresses MBP-tagged ORF24-NTD in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Amino acids 1-201

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX4T1-ORF24-NTD

    Plasmid

    #138464

    Purpose

    Expresses GST-tagged ORF24-NTD in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 1-201

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4/TO-ORF24-3xFLAG

    Plasmid

    #138423

    Purpose

    Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB165 - pL1R3_pMAS::rLUC-I::tMAS

    Plasmid

    #123198

    Purpose

    binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    pMAS::rLUC-I::tMAS
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Xl-VSP1 R152Q GFP

    Plasmid

    #51884

    Purpose

    expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. b2-TS5

    Plasmid

    #103878

    Purpose

    full-length human b2 with tension sensor at b2_743

    Depositor

    Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    full-length human b2 with tension sensor at b2_743 (ITGB2 Human)
    

    Use

    Tags

    tension sensor module

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid

    #153446

    Purpose

    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p6H-SUMO3-ORF24-NTD-Strep

    Plasmid

    #138467

    Purpose

    Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    SUMO3 and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Amino acids 2-201

    Promoter

    T7

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1-zeo-ORF66-2xStrep

    Plasmid

    #130957

    Purpose

    Lentiviral vector for expression of KSHV ORF66 with a C-terminal 2xStrep tag

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

    Plasmid

    #129758

    Purpose

    Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 36 to Alanine and Leucine 3…

    Promoter

    TRE3Gs and Tet inducible promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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