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pDIRECT_25K

Plasmid

#91147

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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FusionRed-BaxAlpha-C-15

Plasmid

#56101

Purpose

Localization: , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

BaxAlpha

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAc5.1B-lambdaN-HA-DmGW182

Plasmid

#22406

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

gawky, variant A (gw Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-hFX-promotor(2,5kb)

Plasmid

#14980

Depositor

Michael Ristow

Insert

human frataxin promoter fragment (FXN Human)

Use

Luciferase

Tags

Expression

Mutation

start codon for human frataxin gene has been muta…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Article

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Use

Tags

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTRANS_263

Plasmid

#91127

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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P199

Plasmid

#139717

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, Luz promoter is circadian controlled

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_pAtELF4:NnLuz:trbcSE9

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtELF4

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-hFX-promotor(1,3kb)

Plasmid

#14979

Depositor

Michael Ristow

Insert

human frataxin promoter fragment (FXN Human)

Use

Luciferase

Tags

Expression

Mutation

start codon for human frataxin gene has been muta…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pDIRECT_21J

Plasmid

#91132

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0301

Plasmid

#91005

Purpose

Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A nickase

Use

CRISPR

Tags

Expression

Mutation

H840A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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acta 2

Plasmid

#78711

Purpose

zebrafish acta2 3-UTR–specific probe

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

acta2 (acta2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

contains bp 1,243–1,591

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
P315-FBP_10

Plasmid

#139701

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_A3910

Plasmid

#91054

Purpose

Module A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

dTaCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0201

Plasmid

#91002

Purpose

Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Tags

Expression

Mutation

D10A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET29NK-LGK/mGFPmut3

Plasmid

#81168

Purpose

GFP fusion to levoglucosan kinase

Depositor

Timothy Whitehead

Article

Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

Insert

Levoglucosan kinase wild-type

Use

Tags

His6x and mGFPmut3

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDIRECT_26K

Plasmid

#91152

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDIRECT_23J

Plasmid

#91142

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22J

Plasmid

#91137

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1

Plasmid

#166846

Purpose

Expresses Atg1 with ATG1 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

ATG1 Promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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