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Showing: 1461 - 1480 of 7326 results

  2. KG#205

    Plasmid
    #110881
    Purpose
    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1
    Inserts
    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Asp 448 to Asn
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110881-plasmid-map-sequence-id-212568

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  6. A2M-bio-His

    Plasmid
    #53423
    Purpose
    Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    A2M (A2M Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53423-plasmid-map-sequence-id-83211

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  12. Human a3 nicotinic receptor subunit EM construct

    Plasmid
    #163960
    Purpose
    pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human alpha3 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Asn348-Ser402 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163960-plasmid-map-sequence-id-323882

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  13. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid
    #101300
    Purpose
    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.
    Depositor
    Alpha Yap
    Article
    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.
    Insert
    alpha-Catenin
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains amino acids 1-509, 670-698, and 699-906 …
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    101300-plasmid-map-sequence-id-246103

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  14. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  16. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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Showing: 1461 - 1480 of 7326 results