We narrowed to 3,816 results for: golden gate

1. Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002

   Plasmid

   #228639

   Purpose

   Bacterial expression of the Coxsackievirus 33 isoform B 3C protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Coxsackievirus 33 isoform B 3C protease
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease

   Plasmid

   #228644

   Purpose

   Bacterial expression of the D68 3C protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Enterovirus D68 3C protease
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pMV_AA101

   Plasmid

   #245323

   Purpose

   Cas9 CRISPRa positive control guide; targets CD274

   Depositor

   John Doench, David Root

   Insert

   sgRNA CD274 (CD274 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRDB_510

   Plasmid

   #245337

   Purpose

   Cas9 CBE positive control guide; targets CD274

   Depositor

   John Doench, David Root

   Insert

   sgRNA CD274 (CD274 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRDB_509

   Plasmid

   #245336

   Purpose

   Cas9 ABE positive control guide; targets CD274

   Depositor

   John Doench, David Root

   Insert

   sgRNA CD274 (CD274 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRDB_248

   Plasmid

   #245335

   Purpose

   Cas12a CRISPRko positive control guide; targets CD63

   Depositor

   John Doench, David Root

   Insert

   sgRNA CD63 (CD63 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTwist-Kan-HC-L0-TaMOFB1(int)

   Plasmid

   #242005

   Purpose

   L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum MORE FLORET 1 (MOF-1) / MULTI-FLORET SPIKELET 2 (MFS-2) B-subgenome homoeolog (TraesCS2B02G420900)

   Depositor

   Cristobal Uauy

   Insert

   TaMOF-B1_with_introns
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAIP4_3CX2

   Plasmid

   #234221

   Purpose

   Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3CX2 (MYBPC3 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pAIP4_4ACJ

   Plasmid

   #234212

   Purpose

   Heterologous protein expression of Wufb25h12 in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4ACJ (oxr1a Zebrafish)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pAIP4_1EYH

    Plasmid

    #234159

    Purpose

    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1EYH (Epn1 Rat)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. MUT_S1-spacer-H1

    Plasmid

    #218399

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HS_NAM-B1

    Plasmid

    #154064

    Purpose

    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Inserts

    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    

    Use

    Cre/Lox and Synthetic Biology; Golden gate

    Expression

    Bacterial and Plant

    Mutation

    The TtNAM-B1 gene sequence was domesticated to re…

    Promoter

    HvHSP17, OsAct, and ZmUbi

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IBMc460

    Plasmid

    #161940

    Purpose

    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    

    Expression

    Bacterial

    Promoter

    PPhlF

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IBMc180

    Plasmid

    #161942

    Purpose

    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAIP4_3RZY

    Plasmid

    #234156

    Purpose

    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3RZY (FABP4 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pMulti_S_ccdB

    Plasmid

    #223853

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Expression

    Bacterial

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRDA_908

    Plasmid

    #245330

    Purpose

    Cas12a CRISPRa positive control guide; targets CD274, ADGRE5, CD4, DPP4

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD274, ADGRE5, CD4, DPP4 (CD274 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pM.L3456

    Plasmid

    #234589

    Purpose

    Linker plasmid (connect pMB.Pos2 to pMB.002)

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Type

    Empty backbone

    Use

    Other

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pM.L56

    Plasmid

    #234591

    Purpose

    Linker plasmid (connect pMB.Pos4 to pMB.002)

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Type

    Empty backbone

    Use

    Other

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pM.L456

    Plasmid

    #234590

    Purpose

    Linker plasmid (connect pMB.Pos3 to pMB.002)

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Type

    Empty backbone

    Use

    Other

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge