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csd5_41-192/pET28b(+)

Plasmid

#83296

Purpose

csd5, construct: 41-192, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1250

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-40

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd4_22-438/pET28b(+)

Plasmid

#83295

Purpose

csd4, construct: 22-438, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1075

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-21

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
p414GPD-3xFLAG-RBM28 WT

Plasmid

#169265

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 WT (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E8 WT minigene

Plasmid

#169271

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLX317-EGFP

Plasmid

#115439

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_IRF3_SMAD/FHA

Plasmid

#178479

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

IRF3_IRF3 (IRF3 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-dCas9-VPR-pgk-hph

Plasmid

#233066

Purpose

Piggybac CRISPRa construct

Depositor

Markus Landthaler

Article

Vucicevic et al Cell Genom. 2025 Oct 8;5(10):100984. doi

Insert

dCas9-VPR

Use

CRISPR

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCas-CmR(+)

Plasmid

#113633

Purpose

Chloramphenicol resistant derivative of pCas plasmid

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

Chloramphenicol resistance gene

Use

CRISPR

Promoter

Pcat

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

Plasmid

#233067

Purpose

Piggybac CRISPRi construct

Depositor

Markus Landthaler

Article

Vucicevic et al Cell Genom. 2025 Oct 8;5(10):100984. doi

Insert

KRAB-dCas9

Use

CRISPR

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pETM41_KEAP1_KELCH

Plasmid

#178476

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KEAP1_KELCH (KEAP1 Human, Synthetic)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer20 Cyclin E1

Plasmid

#109348

Purpose

virus packaged, doxycyline inducible expression of Cyclin E1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cyclin E1 (CCNE1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1-Cdc45

Plasmid

#208796

Purpose

Xenopus laevis FLAG5-His10-TEV-Cdc45

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

Cdc45 (cdc45.L Frog)

Tags

FLAG and His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mCherry

Plasmid

#62635

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-cyclin E

Plasmid

#216508

Purpose

Expresses cyclin E1 in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CCNE1 (CCNE1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-fFuCas9-HTBNLS-hph

Plasmid

#121092

Purpose

Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

Depositor

Xiao-Jun Ji

Article

Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

Insert

Cas9 and hygromycin resist gene

Use

CRISPR

Expression

Bacterial

Promoter

gpdA;trpC

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLacZ-1735

Plasmid

#115454

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shLacZ-1735

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_CRK_SH3

Plasmid

#178472

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CRK_SH3 (CRK Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FLCN

Plasmid

#49173

Purpose

Mammalian expression of mouse folliculin (FLCN) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin (Flcn Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_GFP

Plasmid

#78172

Purpose

Doxycyline regulated expression of GFP in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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