We narrowed to 5,078 results for: mcs

1. JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

   Plasmid

   #232123

   Purpose

   A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

   Plasmid

   #232131

   Purpose

   A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   FOXF2 (FOXF2 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

   Plasmid

   #232130

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 1036 (pME_V5_TCF7L2_P2A_H2A_mCherry)

   Plasmid

   #232126

   Purpose

   A middle entry gateway clone containing V5 tagged human TCF7L2 / TCF4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF7L2 / TCF4 (TCF7L2 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL3342 PB-PEmax-puro

   Plasmid

   #226674

   Purpose

   PiggyBac cargo vector encoding PEmax and puromycin resistance.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   PEmax
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+ dgat1a-FLAG

   Plasmid

   #232143

   Purpose

   Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1a (dgat1a Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRII-TOPO-dgat1b in situ probe

   Plasmid

   #232138

   Purpose

   zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1b
   

   Use

   In vitro transcription

   Promoter

   SP6/T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTBL3328 PB-sfGFP-WPRE-target

   Plasmid

   #226676

   Purpose

   PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   sfGFP-WPRE-site3target
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

9. pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

   Plasmid

   #195567

   Purpose

   PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

   Depositor

   Joanna Wysocka

   Article

   Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

   Insert

   TIR1
   

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL3428 MTK234-LacO-BtoApegRNA

    Plasmid

    #226669

    Purpose

    MTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNA

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    hU6-LacOS-BtoApegRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6-LacOS

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTBL3331 MTK234-TetO-AtoBpegRNA

    Plasmid

    #226668

    Purpose

    MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    hU6-TetO-AtoBpegRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6-TetO

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL3401 BtoApegRNA-sfGFP

    Plasmid

    #226667

    Purpose

    PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    BtoA pegRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-GEM

    Plasmid

    #187648

    Purpose

    Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Insert

    PfV
    

    Use

    AAV

    Tags

    HaloTag and SNAP-tag

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn-tractin

    Plasmid

    #187649

    Purpose

    Expresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Insert

    F-tractin
    

    Use

    AAV

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

    Plasmid

    #214614

    Purpose

    adapter for RTK split TEV recruitment assays

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    3xSH2 domains of GRB2-CTEV (GRB2 Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_ERBB4-NTEV-tcs-GV-2xHA

    Plasmid

    #214613

    Purpose

    ERBB4 receptor for split TEV assay

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    ERBB4-NTEV (ERBB4 Human)
    

    Tags

    TEV-Gal4-VP16-2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3_EGFR-NTEV-tcs-GV-2xHA

    Plasmid

    #214610

    Purpose

    EGFR receptor for split TEV assay

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    EGFR-NTEV (EGFR Human)
    

    Tags

    TEV-Gal4-VP16-2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NK621

    Plasmid

    #196405

    Purpose

    Strong expression of firefly luciferase codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. NK809

    Plasmid

    #196406

    Purpose

    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NK810

    Plasmid

    #196407

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge