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Showing: 3701 - 3750 of 17876 results

  1. ADH1pro_GCR2_SIR4

    Plasmid
    #138619
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCR2 (GCR2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138619-plasmid-map-sequence-id-274988

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  2. pET16b-Prp16

    Plasmid
    #111351
    Purpose
    protein purification
    Depositor
    Christine Guthrie
    Insert
    PRP16
    Use
    Tags
    N-terminally 10xHis-tagged proteins with a Factor…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111351-plasmid-map-sequence-id-217673

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  3. LHP1427 - VPS8

    Plasmid
    #32241
    Depositor
    Linda Hicke
    Insert
    VPS8 (VPS8 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Lac
    Available since
    Oct. 4, 2011
    Availability
    Academic Institutions and Nonprofits only

  4. LHP1021 - BUL1

    Plasmid
    #32439
    Depositor
    Linda Hicke
    Insert
    BUL1 (BUL1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Lac
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  5. pET-19b_MMLV-RT

    Plasmid
    #153312
    Purpose
    Bacterial expression of MMLV reverse transcriptase
    Depositor
    Drew Endy, Philippa Marrack
    Insert
    MMLV Reverse Transcriptase
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153312-plasmid-map-sequence-id-298742

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  6. pWS173

    Plasmid
    #90960
    Purpose
    Cas9 gap repair vector - KanR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90960-plasmid-map-sequence-id-261712

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  7. pOCC102-MBP-eIF4G

    Plasmid
    #219971
    Purpose
    eIF4G protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219971-plasmid-map-sequence-id-436844

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  8. ADH1pro_GCN4_SIR4

    Plasmid
    #138620
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCN4 (GCN4 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    138620-plasmid-map-sequence-id-334951

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  9. p41Nat MagicFluor

    Plasmid
    #58563
    Purpose
    p41Nat carrying both mating type markers buffered by the Cyc1 terminator
    Depositor
    Leonid Kruglyak
    Insert
    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    58563-plasmid-map-sequence-id-90668

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  11. LHP334-pcDNA3-STE2

    Plasmid
    #32158
    Depositor
    Linda Hicke
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. KAP95-FLU (113)

    Plasmid
    #24045
    Depositor
    Michael Rout
    Insert
    KAP95 (KAP95 Budding Yeast)
    Use
    Tags
    FLU
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  13. p41Neo MagicFluor

    Plasmid
    #58564
    Purpose
    p41Neo carrying both mating type markers buffered by the Cyc1 terminator
    Depositor
    Leonid Kruglyak
    Insert
    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    58564-plasmid-map-sequence-id-90669

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  14. KAP123-FLU (116)

    Plasmid
    #24048
    Depositor
    Michael Rout
    Insert
    KAP123 (KAP123 Budding Yeast)
    Use
    Tags
    FLU
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  15. KAP121-FLU (115)

    Plasmid
    #24047
    Depositor
    Michael Rout
    Insert
    KAP121 (PSE1 Budding Yeast)
    Use
    Tags
    FLU
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  16. Sc Rad27-S6- pET24

    Plasmid
    #239205
    Purpose
    Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    Rad27 (RAD27 Budding Yeast)
    Use
    Tags
    S6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239205-plasmid-map-sequence-id-474608

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  17. Sc FLAG-RFC1-pRSFDuet

    Plasmid
    #239201
    Purpose
    Overexpress Sc RFC1 (N-terminal 3X FLAG tag) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    RFC1 (RFC1 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239201-plasmid-map-sequence-id-474602

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  18. pLS400_YCR051W

    Plasmid
    #239129
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX
    Depositor
    Sahand Jamal Rahi
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239129-plasmid-map-sequence-id-474387

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  19. pLS410

    Plasmid
    #239123
    Purpose
    Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX
    Depositor
    Sahand Jamal Rahi
    Type
    Empty backbone
    Use
    Centromeric
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239123-plasmid-map-sequence-id-474392

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  20. pLS418

    Plasmid
    #239128
    Purpose
    Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6
    Depositor
    Sahand Jamal Rahi
    Type
    Empty backbone
    Use
    Centromeric
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239128-plasmid-map-sequence-id-474393

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  21. pRS414-Syf1 817 Truncation

    Plasmid
    #234126
    Purpose
    Syf1 PCR truncation starting at 817 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234126-plasmid-map-sequence-id-466534

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  22. pRS414-Syf1 634 Truncation

    Plasmid
    #234116
    Purpose
    Syf1 PCR truncation starting at 634 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234116-plasmid-map-sequence-id-466457

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  23. pRS414-Syf1 778 Truncation

    Plasmid
    #234115
    Purpose
    Syf1 PCR truncation starting at 778 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234115-plasmid-map-sequence-id-465993

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  24. pOCC102-MBP-Pab1

    Plasmid
    #219979
    Purpose
    Pab1p protein purification
    Depositor
    Simon Alberti
    Insert
    Pab1p (PAB1 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219979-plasmid-map-sequence-id-436849

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  25. pOCC102-MBP-eIF4A

    Plasmid
    #219981
    Purpose
    eIF4A protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4A (TIF1 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219981-plasmid-map-sequence-id-436878

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  26. pOCC22-MBP-eIF4E

    Plasmid
    #219978
    Purpose
    eIF4E protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4E (CDC33 Budding Yeast)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219978-plasmid-map-sequence-id-436833

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  27. pCAS-mak10 gRNA-MAK10Δ

    Plasmid
    #160384
    Purpose
    For CRISPR-mediated deletin og MAK10 in yeast
    Depositor
    John McCusker
    Insert
    MAK10
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    160384-plasmid-map-sequence-id-384173

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  28. PJD1223

    Plasmid
    #160390
    Purpose
    Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT
    Depositor
    John McCusker
    Insert
    L-A Gag-Pol
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TRP1
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160390-plasmid-map-sequence-id-314529

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  29. pFA6a-TRP1-pCUP1-2xphlO

    Plasmid
    #191794
    Purpose
    Yeast gene induction with copper promoter
    Depositor
    Dean Dawson
    Insert
    TRP1 pCUP1 with 2 phlO sites
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191794-plasmid-map-sequence-id-377878

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  30. KBB528

    Plasmid
    #185104
    Purpose
    C-terminal truncation of Pom152 fused to GFP
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    POM152(1-???)-HA-GFP
    Promoter
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185104-plasmid-map-sequence-id-368165

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  31. ADH1pro_GCR1_SIR4

    Plasmid
    #138618
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCR1 (GCR1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138618-plasmid-map-sequence-id-274926

    Enlarge

  32. ADH1pro_CBF1_SIR4

    Plasmid
    #138616
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    CBF1 (CBF1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138616-plasmid-map-sequence-id-275117

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  33. pWS082

    Plasmid
    #90516
    Purpose
    sgRNA entry vector
    Depositor
    Tom Ellis
    Insert
    Yeast sgRNA expression vector
    Use
    CRISPR; Yeast gap repair
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    90516-plasmid-map-sequence-id-197186

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  35. pY113 (pcDNA3.1-huPb2Cpf1)

    Plasmid
    #92272
    Purpose
    Expression of humanized Pb2Cpf1
    Depositor
    Feng Zhang
    Insert
    huPb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92272-plasmid-map-sequence-id-181492

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  36. HCP8

    Plasmid
    #166110
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166110-plasmid-map-sequence-id-330382

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  37. pKS1577

    Plasmid
    #239525
    Purpose
    Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    ScVba2p-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239525-plasmid-map-sequence-id-475912

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  38. pKS1461

    Plasmid
    #239515
    Purpose
    Express the lower portion of melvaonate pathway enzymes (Erg12p, Erg8p, and Erg19p) in the peroxisome; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScErg12p-ePTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239515-plasmid-map-sequence-id-475897

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  39. pKS1575

    Plasmid
    #239523
    Purpose
    Express Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScVba2p-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239523-plasmid-map-sequence-id-475887

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  40. pKS1574

    Plasmid
    #239522
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScYea4p-mTurquoise2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239522-plasmid-map-sequence-id-475877

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  41. pVL1280

    Plasmid
    #239539
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239539-plasmid-map-sequence-id-475864

    Enlarge

  42. Fyv6-NFLAG del103-173

    Plasmid
    #234134
    Purpose
    Amino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Use
    Tags
    Expression
    Yeast
    Mutation
    C-terminus truncation
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234134-plasmid-map-sequence-id-466524

    Enlarge

  43. Fyv6-NFLAG del134-173

    Plasmid
    #234135
    Purpose
    Amino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Use
    Tags
    Expression
    Yeast
    Mutation
    C-terminus truncation
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234135-plasmid-map-sequence-id-466521

    Enlarge

  44. pUC57-T7-PAB1-NLuc

    Plasmid
    #219984
    Purpose
    PAB1-5'UTR-NLuc mRNA synthesis
    Depositor
    Simon Alberti
    Insert
    PAB1-5'UTR-NLuc (PAB1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219984-plasmid-map-sequence-id-436830

    Enlarge

  45. pOCC121-MBP-monoGFP-eIF4G

    Plasmid
    #219972
    Purpose
    eIF4G protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis and MBP-monoGFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219972-plasmid-map-sequence-id-436768

    Enlarge

  46. LDB361

    Plasmid
    #185435
    Purpose
    Bacterial expression of full length NUP2 as fusion with GST
    Depositor
    Laura Davis, Ken Belanger
    Insert
    NUP2
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185435-plasmid-map-sequence-id-367560

    Enlarge

  47. pROS15_MATz

    Plasmid
    #166100
    Purpose
    Expression of dual gRNA targeting the MAT locus
    Depositor
    Rebecca Burgess
    Insert
    MAT Z1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166100-plasmid-map-sequence-id-328822

    Enlarge

  48. pUPD2 CUP2 (GB2716)

    Plasmid
    #160599
    Purpose
    Copper dependent transcription factor
    Depositor
    Diego Orzaez
    Insert
    CUP2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160599-plasmid-map-sequence-id-322067

    Enlarge

  49. LHP428-YCplac33-STE2-378Stop

    Plasmid
    #32172
    Depositor
    Linda Hicke
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Stop codon introduced at aa 378
    Promoter
    STE2
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  50. LHP1048 - pFB-RSP5

    Plasmid
    #32434
    Depositor
    Linda Hicke
    Insert
    RSP5 (RSP5 Budding Yeast)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    P-P10 and P-Pol
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 3701 - 3750 of 17876 results