We narrowed to 4,243 results for: Pet2;

1. pET28-AviTag-DogTag-MBP

   Plasmid

   #171773

   Purpose

   Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogTag-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SENP1 (catalytic domain)

   Plasmid

   #16356

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP1 catalytic domain (SENP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 419 to 644 catalytic domain of human S…

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-TS2126 RNA Ligase

   Plasmid

   #119941

   Purpose

   Express TS2126 RNA ligase in E. coli

   Depositor

   Takashi Ito

   Article

   Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

   Insert

   TS2126 RNA ligase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. WT C-His Ana GvpC in pET28a

   Plasmid

   #85732

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-AviTag-DogCatcher-MBP

   Plasmid

   #171928

   Purpose

   Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogCatcher-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   DogCatcher added to N-terminus of MBP via a GSGES…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZH81 pET21b Rat1-His

   Plasmid

   #231650

   Purpose

   Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   Rat1 (RAT1 Budding Yeast)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-cys-His6-OtUBD

   Plasmid

   #190091

   Purpose

   Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-ptetO::bac::gfp

   Plasmid

   #217882

   Purpose

   expresses the bac-BGC in bacterial cells, produces bacillamide D

   Depositor

   Tobias A. M. Gulder

   Article

   Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

   Inserts

   Dehydrogenase
   Non-ribosomal peptide synthetase
   Aminotransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptetO

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-His-TEV-NQO1

   Plasmid

   #119058

   Purpose

   For expression in E.coli and affinity purification of N-terminally His tagged NQO1

   Depositor

   Yosef Shaul

   Article

   Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

   Insert

   NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
   

   Use

   Tags

   6His, TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SnoopTag-AffiHER2-SpyTag

    Plasmid

    #216280

    Purpose

    Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-AffiHER2-SpyTag
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b-ULP1-405-621

    Plasmid

    #229224

    Purpose

    For expression of ULP1 protein

    Depositor

    Andy LiWang

    Insert

    ULP1 (ULP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    405-621 (truncated)

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a SUMO Nup53xl 1-320

    Plasmid

    #85998

    Purpose

    bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-6XHis-SUMO-SIRT2

    Plasmid

    #102622

    Purpose

    Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Tags

    6XHis-SUMO

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-37 and 357-389

    Promoter

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_mouse-Caspase-1_enzymatic-domain

    Plasmid

    #183385

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404) (Casp1 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-SENP2 (catalytic domain)

    Plasmid

    #16357

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP2 catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 365 to 590 catalytic domain of human S…

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-6His-SUMO-Nsp10

    Plasmid

    #154959

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid

    #129594

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-His6-Ran(Q69L)

    Plasmid

    #171563

    Purpose

    Encodes human Ran protein

    Depositor

    Yan Zeng

    Insert

    Ran (RAN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge