Addgene

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RVD-AT5

Plasmid

#173276

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NI-NG dimer RVD at Position5

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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RVD-AT6

Plasmid

#173277

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NI-NG dimer RVD at Position6

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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RVD-AT7

Plasmid

#173278

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NI-NG dimer RVD at Position7

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-pchD WT

Plasmid

#187973

Purpose

Expresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Shelton et al J Biol Inorg Chem. 2022 May 5. pii: 10.1

Insert

pchD WT

Use

Tags

Hexahistag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H

Plasmid

#187188

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

D34R, D87R

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H

Plasmid

#187184

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

L32F, L33F

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H

Plasmid

#187185

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Y90A, V91A

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H

Plasmid

#187183

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

L32A, L33A

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-Alox12-PLAT-EGFP-6H

Plasmid

#187190

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000069463 (alox12 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-PKCgamma-C2-EGFP-6H

Plasmid

#187115

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000126583 (PRKCG Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H

Plasmid

#187187

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

D34K, D87K

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

Plasmid

#187186

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Y90F, V91F

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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RVD-hC9

Plasmid

#173319

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

HD half RVD at Position9

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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RVD-AG9

Plasmid

#173268

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NI-NN dimer RVD at Position9

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Lentiguide-Blast-eNMU710

Plasmid

#172658

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-eNMUA/pegRNA-eNMUB

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PB-HD252

Plasmid

#172656

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD252A/pegRNA-HD252B

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGH50

Plasmid

#107254

Purpose

Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

vipp1-gfp-fkbp12

Use

Suicide vector for destined for double homologous…

Tags

GFP and FKBP12

Expression

Bacterial

Mutation

Promoter

native

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC S431A T432A NS1

Plasmid

#101837

Purpose

drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431A T432A with p0050 promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

S431A T432A

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC S431E T432E NS1

Plasmid

#101836

Purpose

drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431E T432E with p0050 promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

S431E T432E

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRAF-MutL(R95F)

Plasmid

#86608

Purpose

E. coli Mutl(R95F) expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutL(R95F)

Use

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

R95F

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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