We narrowed to 9,590 results for: ef1;

1. pLV-EF1A-CD19.8H.28TM.28.3z

   Plasmid

   #200674

   Purpose

   Modular CD8hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD28TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICOZ-SB100X[EF1α-CD19 4-1BB]

   Plasmid

   #218328

   Purpose

   Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   CD19 CAR mammalian expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP453 pEf1a-NLS-aGCN4-mCherry

   Plasmid

   #100945

   Purpose

   mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, mCherry
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-fDIO-stGtACR2-FusionRed

   Plasmid

   #217975

   Purpose

   AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

   Depositor

   Edward Callaway

   Article

   Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

   Insert

   stGtACR2-FusionRed
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-CVS-G-WPRE-pGHpA

   Plasmid

   #67528

   Purpose

   "Expresses the rabies glycoprotein"

   Depositor

   Botond Roska

   Article

   Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

   Insert

   Rabies virus strain CVS-11 glycoprotein (G)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FRT-EF1a-TVA-2A-N2cG

   Plasmid

   #172361

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-OMOR-eYFP

   Plasmid

   #66847

   Purpose

   Expresses Opto-MOR in a Cre dependent manner

   Depositor

   Michael Bruchas

   Article

   Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

   Insert

   Opto Mu opioid receptor
   

   Use

   AAV and Cre/Lox

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX-EF1a ANXA11-mEmerald D40G

   Plasmid

   #164211

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   D40G

   Promoter

   EF1a

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1alpha-EPO-218-PDGFR

   Plasmid

   #139057

   Purpose

   A lentivirus vector to integrate a surface membrane anchored human EPO gene

   Depositor

   Tao Liu

   Article

   Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

   Insert

   erythropoietin(EPO) (EPO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-FHC-POLQ-K121M

    Plasmid

    #64877

    Purpose

    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M, a mutation in the conserved ATP-binding si…

    Promoter

    EF1alpha

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-EF1a-HygroR-T2A-TetOn3G

    Plasmid

    #179887

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    Hygromycin resistance

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV EF1a DIO iChloC 2A dsRed

    Plasmid

    #70762

    Purpose

    An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    EF1a

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-DIO-tdTomato-WPRE

    Plasmid

    #202610

    Purpose

    Double floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

    Plasmid

    #170220

    Purpose

    Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T14A, Y15F

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-IRES-Puro PFKM

    Plasmid

    #201638

    Purpose

    Over-expression of untagged human PFKM

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PFKM (PFKM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1-FHC-POLQ-DY2230AA

    Plasmid

    #64878

    Purpose

    lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    D2330A,Y2331A, in the DNA polymerase domain (POL)…

    Promoter

    EF1alpha

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-EF1a-GFP-miRE_shRen-Puro

    Plasmid

    #135683

    Purpose

    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-Con/Fon-sRGECO

    Plasmid

    #137126

    Purpose

    Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-sRGECO
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E217D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-EF1aL-Cre-IRES-NeoR-W

    Plasmid

    #126700

    Purpose

    lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    Cre-IRES
    

    Use

    Cre/Lox and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-Coff/Fon-GCaMP6M

    Plasmid

    #137121

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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