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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,050 results for: erf

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Showing: 1481 - 1500 of 3050 results

  2. pFP35

    Plasmid
    #247210
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (DF30ppr) from a T7 promoter. Suitable for cell-free expression.
    Depositor
    Elizabeth Strychalski
    Publication
    Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with F30 scaffold)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    247210-plasmid-map-sequence-id-507798

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  3. ER2-hfYFP

    Plasmid
    #186518
    Purpose
    Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hyperfolder YFP
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186518-plasmid-map-sequence-id-368092

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  4. pCMV-HA-mIFITM3-K24,83,88,104A

    Plasmid
    #58408
    Purpose
    Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)
    Depositor
    Howard Hang, Jacob Yount
    Publication
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lysines 24, 83, 88, and 104 to Alanine
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. spa-GCaMP6f

    Plasmid
    #67558
    Purpose
    photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Publication
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spa-GCaMP6f
    Tags
    HIS-tag
    Expression
    Mammalian
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    CMV
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pKb-POC1-CBE

    Plasmid
    #255131
    Purpose
    Plant expression of A3A(Y130F)-OpenCRISPR1 D10A-UGI for cytosine base editing.
    Depositor
    Kutubuddin Molla
    Publication
    Das et al New Phytol. 2026 May 21. doi: 10.1111/np
    Insert
    A3A(Y130F) fused at 5' of the Rice codon optimized pOC1 D10A and UGI at the 3' end
    Tags
    NA
    Expression
    Plant
    Promoter
    Maize ubiquitin promoter for A3A(Y130F)-OpenCRISP…
    Available since
    July 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    255131-plasmid-map-sequence-id-516879

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  8. F-IRF7

    Plasmid
    #169777
    Purpose
    Express IRF7 in mammalian cells
    Depositor
    Sung-Liang Yu
    Publication
    Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)
    Tags
    Flag tag
    Expression
    Bacterial and Mammalian
    Available since
    July 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    169777-plasmid-map-sequence-id-334993

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  12. NK-1 GFP

    Plasmid
    #24508
    Depositor
    Ron Vale
    Publication
    Case et al Cell. 1997 Sep 5. 90(5):959-66.
    Insert
    Drosophila NCD, human kinesin
    Tags
    GFP and His
    Expression
    Bacterial
    Mutation
    NK-1 is a chimera where the entire superfamily-co…
    Available since
    June 30, 2010
    Availability
    Academic Institutions and Nonprofits only

  17. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

    Plasmid
    #196086
    Purpose
    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA targeting lncRNA NORAD
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Cloned using SapI sites - destroyed after inserti…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196086-plasmid-map-sequence-id-388997

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  18. pcDNA3-sACE2v5-sfGFP

    Plasmid
    #145176
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 5
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145176-plasmid-map-sequence-id-292575

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  19. pcDNA3-sACE2v4-sfGFP

    Plasmid
    #145175
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 4
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145175-plasmid-map-sequence-id-292620

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Showing: 1481 - 1500 of 3050 results