We narrowed to 1,670 results for: nif

1. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

   Plasmid

   #101213

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K855A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K855A

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKMW306_U6-sgRNA-EMX1

   Plasmid

   #244824

   Purpose

   Mammalian expression of SpyCas9 single guide RNA targeting EMX1

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 single guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKMW124_dCas9

   Plasmid

   #244821

   Purpose

   Mammalian expression of catalytically inactive Cas9 (dCas9)

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   dCas9 (WT)
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS

   Plasmid

   #244842

   Purpose

   Bacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Flex-Cas12a
   

   Use

   CRISPR

   Tags

   10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

   Expression

   Bacterial

   Mutation

   G146R/R182V/D535G/S551F/D665N/E795Q

   Promoter

   T7

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS26_10xHis_MBP_TEV_VRER-Cas9

   Plasmid

   #244829

   Purpose

   Bacterial expression of VRER Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   VRER Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   D1135V/G1218R/R1335E/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHS16_10xHis_MBP_TEV_xPBA-Cas9

   Plasmid

   #244830

   Purpose

   Bacterial expression of xPBA Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   xPBA Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS

   Plasmid

   #244833

   Purpose

   Bacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 2xSV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHS23_10xHis_MBP_TEV_SpG-Cas9

   Plasmid

   #244826

   Purpose

   Bacterial expression of SpG Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpG Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHS1_10xHis_MBP_TEV_WT-Cas9

   Plasmid

   #244825

   Purpose

   Bacterial expression of SpyCas9 (WT) for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHS25_10xHis_MBP_TEV_EQR-Cas9

    Plasmid

    #244828

    Purpose

    Bacterial expression of EQR Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    EQR Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135E/R1335Q/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHS9_10xHis_MBP_TEV_dCas9

    Plasmid

    #244831

    Purpose

    Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    dCas9 (WT)
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D10A/H840A

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a asyn(66-140) E83A

    Plasmid

    #240583

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a asyn(66-140) K80A

    Plasmid

    #240584

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K80A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-7 asyn K97A

    Plasmid

    #240601

    Purpose

    Overexpress mutant alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    K97A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a asyn(66-140) K96A

    Plasmid

    #240585

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K96A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-7 asyn K96A

    Plasmid

    #240600

    Purpose

    Overexpress mutant alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    K96A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a asyn(66-140) K97A

    Plasmid

    #240586

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K97A

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a asyn(66-140) K102A

    Plasmid

    #240588

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K102A

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a asyn(66-140) D98A

    Plasmid

    #240587

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, D98A

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-7 asyn K80A

    Plasmid

    #240599

    Purpose

    Overexpress mutant alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    K80A

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge