We narrowed to 2,328 results for: plasmid for e coli
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Plasmid#134925PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.DepositorInsertCascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
ExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA
Plasmid#134924PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
ExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2870
Plasmid#232515PurposeGenetic complementation plasmid of Cys-light LetA(L94C,I383C) with LetBDepositorExpressionBacterialMutation[LetA1(1-223, L94C,C124S)]-[LetA2(224-427,I383C,C…PromoterT7Available SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2871
Plasmid#232516PurposeGenetic complementation plasmid of Cys-light LetA(Q180C,R380C) with LetBDepositorExpressionBacterialMutation[LetA1(1-223,Q180C,C124S)]-[LetA2(224-427,R380C,C…PromoterT7Available SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2874
Plasmid#232517PurposeExpression plasmid of Cys-light LetA(L94C) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutation[6xHis2xQH-TEV-LetA1(1-223,L94C,C124S)]-[LetA2(22…PromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2875
Plasmid#232518PurposeExpression plasmid of Cys-light LetA(Q180C) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutation[6xHis2xQH-TEV-LetA1(1-223,Q180C,C124S)]-[LetA2(2…PromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2889
Plasmid#232521PurposeExpression plasmid of Cys-light LetA(I383C) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutation[6xHis2xQH-TEV-LetA1(1-223,C124S)]-[LetA2(224-427…PromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2890
Plasmid#232522PurposeExpression plasmid of Cys-light LetA(R380C) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutation[6xHis2xQH-TEV-LetA1(1-223,C124S)]-[LetA2(224-427…PromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2809
Plasmid#232510PurposeExpression plasmid of Cys-light LetA(L94C,I383C) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutation[6xHis2xQH-TEV-LetA1(1-223,L94C,C124S)]-[LetA2(22…PromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2820
Plasmid#232511PurposeExpression plasmid of Cys-light LetA(Q180C,R380C) with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutation[6xHis2xQH-TEV-LetA1(1-223,Q180C,C124S)]-[LetA2(2…PromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2802
Plasmid#232509PurposeExpression plasmid of Cys-light LetA with LetBDepositorTags6xHis2xQH-TEVExpressionBacterialMutation[6xHis2xQH-TEV-LetA1(1-223,C124S)]-[LetA2(224-427…PromoteraraBADAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMM643
Plasmid#112533PurposepZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
ExpressionBacterialMutationA17PPromoterJ23107, PL(HrtO), and ProDAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ABE-NT-sgRNA
Plasmid#112734PurposeAAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNADepositorInsertABE-NT-sgRNA
UseAAVAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
TagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCasTet-λ
Plasmid#128318PurposeConstitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.DepositorInsertTetR and pTetR/TetO
UseCRISPR and Synthetic BiologyExpressionBacterialMutationConstitutive expression of cas9 and anhydro-tetra…Promotertet promoterAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2355_Tier3(PB)-iRFP_PuroR
Plasmid#169654PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2340_Tier3(SB)-iRFP_Puro
Plasmid#169638PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2362_Tier3(SB)-rtTA-PuroR
Plasmid#169658PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2353_Tier3(PB)-YPet_PuroR
Plasmid#169652PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only