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pMJ326: pCMV-GtFz1

Plasmid

#205264

Purpose

Human expression vector for human codon optimized GtFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Kan)

Plasmid

#110089

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXNH3

Plasmid

#110079

Purpose

Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCOND (Apr)

Plasmid

#110094

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Hyg)

Plasmid

#110090

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCOND (Hyg)

Plasmid

#110093

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCR8/GW:PtU6

Plasmid

#104895

Purpose

Phaeodactylum U6 promoter

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

Phaeodactylum U6 promoter

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Apr)

Plasmid

#110091

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKOdeltasacB (Kan)

Plasmid

#110092

Purpose

Analogous to pKO but without the negative selection gene sacB.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-AO7/2537

Plasmid

#11470

Depositor

Allan Weissman

Article

Lorick et al Proc Natl Acad Sci U S A. 1999 Sep 28. 9

Insert

AO7 (Rnf25 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

n/a

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
MbCas12a_K487_GPG_GR2

Plasmid

#231577

Purpose

Encodes a cortisol inducible variant of MbCas12a

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

MbCas12a with a GR2 insertion behind K487

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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MbCas12a_N1153_GPG_GR2

Plasmid

#231578

Purpose

Encodes a cortisol inducible variant of MbCas12a

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

MbCas12a with a GR2 insertion behind N1153

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pZZMP132

Plasmid

#231757

Purpose

Mammalian expression of SPO11-EA

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-EA (Spo11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

E224A

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJJB1426

Plasmid

#218622

Purpose

Express tNCS to the peroxisome for toxicity assay

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

tNCS

Use

Tags

YFP-ePTS1

Expression

Yeast

Mutation

106-588

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1462

Plasmid

#218623

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Use

Tags

ePTS1

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1463

Plasmid

#218624

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Use

Tags

ePTS1

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1734

Plasmid

#218627

Purpose

Express amorphadiene synthase (ADS)

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

ADS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1535

Plasmid

#218613

Purpose

Express Pex5

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1536

Plasmid

#218617

Purpose

Express Pex5 and Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1376

Plasmid

#218618

Purpose

Express Pex5, Pex3, Pex19, and Pex8

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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