We narrowed to 2,475 results for: T2A

1. pPac-PL-mCD8-P2A-Gal4

   Plasmid

   #39460

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   mCD8-P2A-Gal4
   

   Promoter

   actin

   Available Since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPac-PL-mCD8-Gal4

   Plasmid

   #39456

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   mCD8-Gal4
   

   Use

   S2

   Promoter

   actin

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPac-PL-mCD8-E2A-Gal4

   Plasmid

   #39458

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   mCD8-E2A-Gal4
   

   Promoter

   actin

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPac-PL-mCD8-F2A-Gal4

   Plasmid

   #39459

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   mCD8-F2A-Gal4
   

   Promoter

   actin

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL7 lenti-dcas9-3xflag-blast

   Plasmid

   #112133

   Purpose

   lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   dCas9(D10A, N863A)-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A mutants in Cas9

   Promoter

   EF1a

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC-attB-burs-mCD8-EGFP-Gal4

   Plasmid

   #39482

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   burs-mCD8-EGFP-Gal4
   

   Promoter

   bursalpha

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-DRGFP

   Plasmid

   #113193

   Purpose

   DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

   Depositor

   Adam Karpf

   Insert

   DR-GFP
   

   Use

   Donor construct with t2a-hygromycin for gene trap…

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TO-MYOD1-shOct4

   Plasmid

   #182309

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   MYOD1-shOct4 (MYOD1 Human)
   puro-T2A-mycNLS-mTagBFP2
   

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   shOct4

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJW1511

   Plasmid

   #62365

   Purpose

   pEF1a-puro-T2A-ATHmmuRPL10A

   Depositor

   Jonathan Weissman

   Article

   Jan et al Science. 2014 Nov 7;346(6210):1257521. d

   Insert

   Puro-T2A-AVITEVHAmmuRPL10A
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204722

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZHY013

    Plasmid

    #36185

    Depositor

    Daniel Voytas

    Article

    Zhang et al Plant Physiol. 2012 Nov 2.

    Insert

    Heterodimeric FokI nuclease
    

    Use

    Entry vector for expression in plants as a t2a li…

    Promoter

    none

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYL116

    Plasmid

    #239967

    Purpose

    CRISPR donor plasmid with Puro-T2A-mCherry cassette for Suz12 disruption

    Depositor

    Thomas Cech

    Article

    Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

    Insert

    PuroR-T2A-mCherry with homology arms for human Suz12
    

    Use

    CRISPR

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYL117

    Plasmid

    #239968

    Purpose

    CRISPR donor plasmid with Puro-T2A-mCherry cassette for EZH2 disruption

    Depositor

    Thomas Cech

    Article

    Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

    Insert

    PuroR-T2A-mCherry with homology arms for human Ezh2
    

    Use

    CRISPR

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204729

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204727

    Purpose

    Frt-inducible CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

    Plasmid

    #204724

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDRM209 LTP (Luciferase tdTomato Puro)

    Plasmid

    #174723

    Purpose

    Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    tdTomato
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and T2A linker

    Expression

    Mammalian

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Antonella Fidanza, Lesley Forrester

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-DNMT3A-PuroR_hNT

    Plasmid

    #71830

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    Non-targeting sgRNA human (DNMT3A Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid

    #71828

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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