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pJDC182

Plasmid

#60854

Purpose

Expresses firefly luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Firefly luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pPS1622

Plasmid

#8854

Depositor

Pamela Silver

Article

Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

Insert

Sec63-sGFP (SEC63 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

sGFP=S65T V163A mutant of GFP

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
EF.hICN1

Plasmid

#113715

Purpose

Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL

Plasmid

#101002

Purpose

Construct for fusion expression of GeNL reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCM-HA-Gas1

Plasmid

#38313

Depositor

Susan Lindquist

Article

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

Gas1 (GAS1 Budding Yeast)

Tags

2xHA

Mutation

contains promoter region

Promoter

Endogenous

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pRSETb-pHlash

Plasmid

#61550

Purpose

Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification

Depositor

Carl Johnson

Article

Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

Insert

pHlash

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCNPpo6-H98A

Plasmid

#46087

Depositor

Raymond Monnat

Article

Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

Insert

PpoI H98A

Tags

NLS

Expression

Mammalian

Mutation

catalytically inactive H98A variant

Promoter

CMV

Available Since

June 21, 2014

Availability

Academic Institutions and Nonprofits only

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jk089_h12

Plasmid

#112151

Purpose

C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

C. thermophilum DASH/Dam1 complex

Tags

his tag on Spc34 and strep tag on Dad1

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfPar3_3

Plasmid

#26705

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Par3 RNAi

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
BB1_O_34_AMA1_2.8

Plasmid

#90293

Purpose

BB1 with AMA1_2.8 sequence for transient plasmid propagation in A. niger between FS 34

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

AMA1_2.8

Expression

Bacterial

Mutation

it is a truncated version of AMA1 (5.3 kb)

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8

Plasmid

#90292

Purpose

BB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistance

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mttA

Use

A niger

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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DE_Thr6XHisFLAG

Plasmid

#139949

Purpose

Protein tags

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thrombin6XHisFLAGStop

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-CtDCP2_1-267_X

Plasmid

#148004

Purpose

Bacterial Expression of CtDCP2_1-267

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP2_1-267

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1_925-2193_X

Plasmid

#147972

Purpose

Bacterial Expression of CtNot1_925-2193

Depositor

Elisa Izaurralde

Article

Raisch et al J Struct Biol. 2018 Dec;204(3):388-395.

Insert

CtNot1_925-2193

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+FFluc

Plasmid

#26156

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Firefly luciferase

Expression

Bacterial

Mutation

Firefly luciferase gene codon optimised for mycob…

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET15b-AavLEA1

Plasmid

#53093

Purpose

AavLEA1 expression (in BL21 DE3 E. coli strain)

Depositor

Claude Férec

Article

Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

Insert

AavLEA1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZapCY1

Plasmid

#36319

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ZapCY1 high affinity Zn(II) sensor

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZapCY2

Plasmid

#36320

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ZapCY2 high affinity Zn(II) sensor

Expression

Mammalian

Mutation

see comment

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only
NES-EGFP-P4Mx1

Plasmid

#108121

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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