We narrowed to 8,785 results for: sgrna

1. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

   Plasmid

   #207878

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM040 [Multiome Perturb-seq sgRNA expression vector]

   Plasmid

   #224898

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

   Depositor

   Thomas Norman

   Article

   Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

   Plasmid

   #207880

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

   Plasmid

   #221233

   Purpose

   Express sgRNA targeting HEK3 loci with nCas9(D10A)

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

   Plasmid

   #107407

   Purpose

   Lentiviral expression of gRNA resistant PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   sg resistant PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   sgRNA resistant

   Promoter

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEM318 - sgRNA mosTI unc-119 single-copy

   Plasmid

   #159822

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 single-copy
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR

   Plasmid

   #154001

   Purpose

   Vector expressed CasRx and sgRNA_Ptbp1 for AAV package

   Depositor

   Hui Yang

   Article

   Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

   Insert

   CasRx, Ptbp1 sgRNAs
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   GFAP, U6

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEM320 - sgRNA mosTI unc-119 array insertion

   Plasmid

   #159824

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 array insertion
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid

    #159086

    Purpose

    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

    Depositor

    Jeremy Day

    Article

    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

    Insert

    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN, U6

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

    Plasmid

    #118594

    Purpose

    Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    sgRNA/Puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

    Plasmid

    #166873

    Purpose

    AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Inserts

    U6-VEGFA array presgRNA
    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

    Plasmid

    #188703

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

    Plasmid

    #211687

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

    Plasmid

    #136270

    Purpose

    Expresses ABE and ST2-com modified sgRNA scaffold

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Insert

    ABE and sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid

    #132560

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

    Plasmid

    #86195

    Purpose

    U6 based expression of N meningitidis sgRNA

    Depositor

    Scot Wolfe

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    nmCas9 sgRNA cloning cassete
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #239608

    Purpose

    Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    filler-trcr-H1-filler
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231367

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231368

    Purpose

    Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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