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pHH0103_ACTG2_Actin_2

Plasmid

#109990

Purpose

Protein expression and purification of ACTG2_Actin_2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ACTG2_Actin_2 (ACTG2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pmiCas9-msa

Plasmid

#164833

Purpose

miCas9-msa fusion protein expressing plasmid

Depositor

Eugene Chen

Article

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miCas9-msa

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

contains mSA sequence range is 5106-5447 and Brex…

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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PPIB

Plasmid

#155838

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPIB (PPIB Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-u(M)Cas9

Plasmid

#127384

Purpose

Expresses Cas9 at medium level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(M)-Cas9

Use

CRISPR

Expression

Insect

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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PBE-NG

Plasmid

#229045

Purpose

Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.

Depositor

Huawei Zhang

Article

Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESRRA_Hormone-recep

Plasmid

#109908

Purpose

Protein expression and purification of ESRRA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRA_Hormone-recep (ESRRA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST SNAP29-V5

Plasmid

#69821

Purpose

Expresses SNAP29-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Synaptosomal-associated protein 29 (SNAP29 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Promoter

cmv

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-lincRNA-RoR

Plasmid

#45763

Depositor

George Daley

Article

Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

Insert

lincRNA-RoR cDNA (LINC-ROR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2_SH3

Plasmid

#110015

Purpose

Protein expression and purification of GRB2_SH3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GRB2_SH3 (GRB2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

Plasmid

#170175

Purpose

Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2f-ST-P2A-NLS-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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THOC1

Plasmid

#156081

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC1 (THOC1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-u(S)Cas9

Plasmid

#127383

Purpose

Expresses Cas9 at high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(S)-Cas9

Use

CRISPR

Expression

Insect

Promoter

UAST

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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SYMPK

Plasmid

#156071

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SYMPK (SYMPK Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#170161

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME-P2A-H2B-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SR140

Plasmid

#156037

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SR140 (U2SURP Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB1_Guanylate-kin

Plasmid

#110036

Purpose

Protein expression and purification of CACNB1_Guanylate-kin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CACNB1_Guanylate-kin (CACNB1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1_LIM

Plasmid

#109780

Purpose

Protein expression and purification of LASP1_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LASP1_LIM (LASP1 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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PRPF18

Plasmid

#155846

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF18 (PRPF18 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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